Chromatographic purification method

ABSTRACT

A chromatographic purification method for the isolation of a desired product fraction from a mixture using 2 chromatographic columns, comprises, within one cycle to be carried out at least once, the following steps: a first batch step, wherein said columns are disconnected and a first column is loaded with feed and its outlet is directed to waste, and from a second column desired product is recovered and subsequently the second column is regenerated; a first interconnected step, wherein the outlet of the first column is connected to the inlet of the second column, the first column is loaded beyond its dynamic breakthrough capacity with feed, and the outlet of the second column is directed to waste, a second batch step analogous to the first batch step but with exchanged columns; and a second interconnected step, analogous to the first interconnected step but with exchanged columns.

TECHNICAL FIELD

The present invention relates to methods of chromatographic purification, in particular using affinity chromatography, and in particular for capture chromatography, to methods for setting up such processes and to methods for the control of such processes.

PRIOR ART

Capture chromatography is used in the manufacturing of biopharmaceuticals, pharmaceuticals, nutraceuticals, chemicals and other products of interest as initial purification step following the upstream production. The main aim of capture chromatography is the concentration of the product with high throughput and high recovery/yield. In the ideal case a large impurity clearance is obtained simultaneously.

For capture chromatography of biopharmaceuticals preferably columns packed with affinity chromatography stationary phases are used. Affinity materials offer a very high selectivity for the target molecules as they are based on immobilized ligands that bind specifically to the target molecules while letting impurities pass by unaffected. Due to the comparably high manufacturing costs of the affinity ligands, which are typically very elaborate, affinity materials are among the most expensive stationary phases available.

Traditionally, capture chromatography is run in single column, discontinuous mode which is characterized in that the column is consecutively (i) equilibrated, (ii) loaded with feed containing the target product, (iii) washed, (iv) eluted for recovering the desired target product, (vi) cleaned, and (i) re-equilibrated for the next run. The possible maximum load of the column with product is strongly dependent on the stationary phase capacity. A distinction has to be made between the static capacity, which corresponds to the occupancy of all ligands with target molecules obtained under equilibrium conditions (infinite contact time between ligands and target molecules), and the dynamic binding capacity under flow conditions (limited contact time between ligands and target molecules). The dynamic binding capacity depends among other factors on the linear flow rate and mass transfer properties.

Dynamic capacities are typically determined by measuring a so called breakthrough curve, i.e. the concentration curve over time or volume of the product of interest at the column outlet during continued load. In many cases the product concentration can be determined online at that outlet, typically by measuring UV light absorbance. In order to determine the completeness of the breakthrough (i.e. the load value where the outlet concentration reaches the feed concentration, also called saturation point) from the online detector signal it is useful to determine the feed signal as reference using the same detector in the absence of the column.

Depending on the abovementioned factors, the dynamic binding capacity is typically significantly lower than the static binding capacity. This entails that under flow conditions in single column chromatography, the stationary phase capacity is not fully and efficiently utilized since the loading has to be stopped far before the static capacity is reached in order to avoid product losses. On the one hand, the higher the linear loading flow rate is set, the higher the throughput becomes, but the lower the available dynamic binding capacity becomes, leading to early product breakthrough and lower effective stationary phase capacity utilization. On the other hand, the lower the linear flow rate is set, the higher the capacity utilization becomes but the lower the throughput.

Dual loading flow strategies for single column chromatography have been presented that represent a compromise between the two directions. These strategies comprise starting to load the column with a high flow rate in order to maintain high throughput, and then reducing the flow rate in order to obtain high capacity utilization and show advantages over the loading with a uniform flow rate.

Following the loading step, the column is washed, eluted and cleaned, even though the stationary phase has not been fully utilized. Since column cleaning is a major cause for stationary phase degradation the column packing has to be replaced after a certain number of cycles. Consequently, the stationary phase costs with respect to the amount of product produced are significantly larger under dynamic conditions than they would be if the full capacity of the stationary phase (i.e. the static binding capacity) was exploited.

In order to increase the stationary phase capacity utilization and increase the process productivity also the concept of continuous countercurrent chromatography has found application and a number of processes have been adapted from the chemical industry where the concept has been used for a longer time.

Most processes suggest the use of multiple identical columns that are loaded in sequence during the feed step. The motivation for this approach is that during the feeding step the parts of the column that are closer to the column inlet are in contact with higher product concentrations for a longer time and are therefore utilized better (i.e. they are closer to equilibrium corresponding to static conditions) than the parts of the column that are closer to the column outlet. Thus, when multiple short sequentially connected columns are loaded instead of a single long column the most upstream column which has the highest stationary phase utilization can be eluted and cleaned independently of the downstream columns that are loaded to a much lesser extent at the end of the loading step. Therefore only stationary phase with high capacity utilization is eluted and subsequently cleaned. While the fully loaded column is eluted and cleaned, the sequential loading progresses with the next column. Once the formerly upstream column has completed the elution and regeneration steps, it is coupled to the sequentially loaded columns at the most downstream position. Thus, by eluting only columns that have been loaded up to their static capacity, the product amount produced per stationary phase cycle is maximized.

Based on this principle, for the purification of therapeutic proteins from cell culture harvest, a number of multicolumn sequential loading processes have been described, among those processes with up to six columns. The application of simulated moving bed chromatography with eight columns for the capture of monoclonal antibodies from cell culture harvest using Protein A chromatography has even been described.

Sequential loading processes are not limited to continuous upstream manufacturing but may be likewise applied to batch-wise upstream manufacturing. In this case, process control is facilitated by the fact that most products are stable and that the product concentration in the feed is constant since the upstream process is completed before the start of the downstream purification process.

In continuous upstream manufacturing of biopharmaceuticals that is operated for a longer time period (days, weeks, months) a fluctuation of the feed concentration is typical, which demands appropriate process analytical tools and control strategies for the downstream sequential loading processes. Moreover the gradual decline of column capacity with the number of cycles requires the use of these tools and control strategies. Control strategies for controlling flow rates and durations of the chromatographic steps of other types of cyclically multicolumn continuous processes have been suggested based on peak retention times, inline analysis, and at-line analysis, respectively.

A control method for sequential loading processes based on continuously determining the binding capacity of each column in cyclic steady state has been described in WO2010/151214. The method comprises measuring a feed signal, representative of the composition of a feed material supplied to the inlet of the column, measuring an effluent signal representative of the composition of the effluent from the column, and using the feed signal and the effluent signal, to determine binding capacities from a so called “delta signal”. A major drawback of this method is that the feed signal is determined using a first detector and the effluent signal is determined using a second detector. Despite the detectors being of the same type, the detectors must be calibrated accurately and on a regular basis in order to derive useful information from the delta signal such as breakthrough and saturation points that are ultimately used to derive control actions. The method requires the determination of a feed signal by a separate detector whose only purpose is the determination of the feed signal. The number of required detectors is therefore equal to the number of columns+1. The method cannot be used in cases the breakthrough curves cannot be accurately monitored, for instance due to a high impurity signal or due to a low product signal in the feed or both.

Continuous processes and their control align perfectly with the ongoing initiatives of health administrations, which strongly encourage the development and use of process analytical tools for understanding and controlling pharmaceuticals production.

SUMMARY OF THE INVENTION

The present invention relates to countercurrent chromatography processes as well as methods for design, setup, control and optimization of sequential countercurrent loading chromatography processes.

It was surprisingly found that by an innovative combination of the advantages of the sequential loading chromatography, reducing the number of columns to two, applying a dual loading flow strategy and, if needed, applying a process control strategy using the same detector to record the relevant signals for the control, a novel process including design and control methods could be developed exhibiting excellent productivity and capacity utilization performance. The twin-column sequential loading process requires significantly less hardware (valves, detectors, fluid connections, columns) than sequential loading processes with three or more columns.

The control method is not only applicable to methods involving only two columns, but is also and equivalently applicable to twin-, three- and multicolumn countercurrent capture processes where at least two columns are loaded sequentially (connected in series) in one phase of the cyclic process and at least one column is loaded in a second phase of the process. So the control method is to be seen in the context of the above two column process, but also, and quite independently, also in the context of other countercurrent processes with two or more columns. The described control methods require for each chromatographic column a detector to be mounted such that it is capable of recording a signal at the outlet of the column corresponding to the column effluent.

Chromatographic Capture Method:

As a first aspect of the invention, a method of chromatographic purification is proposed using a countercurrent sequential loading chromatography process as schematically illustrated in FIG. 1 with two chromatographic columns only. In the figure, interconnected phases are denoted with IC, disconnected (batch) phases are denoted with B. Interconnected phase means a step where the columns are in a serial connection, i.e. the outlet of an upstream column is connected to the inlet of a downstream column. Disconnected (batch) phase means a step where the columns are not connected but individually loaded with feed or just with solvent via their inlets and their outlets are directed to waste or product collection. A cycle of the process comprises two switches, wherein each switch in turn comprises one B phase and one IC phase. During the B phase of the first switch, column 1 is loaded with feed while column 2 is eluted (product recovery) and regenerated, with regeneration including, if needed, preloading with feed as detailed below.

During the loading of column 1 the flow rate can be kept constant or, according to a preferred embodiment, it is possible to start with a high flow rate and according to a desired profile to reduce the flow rate towards the end of the loading of column 1 in this step (e.g. by using a hyperbolic profile). Or it is possible, in a first phase of this loading, to have a high flow rate, and in a second final phase, a lower flow rate (single or multiple step function). It is possible to use loading flow rates that are approximately at most 2-fold larger than the maximum loading flow rate of the interconnected phase since the columns are operated in disconnected mode, which reduces the chromatographic bed height and thereby the back-pressure by approximately a factor of 2 compared to the interconnected phase. This allows for a fast loading and an efficient initial phase and a slow final phase of the loading process with improved chromatographic mass transfer, leading to an optimum product profile in the column in an optimum amount of time.

The product recovery and regeneration phase with column 2 implies the elution of the desired product (recovery) and preparation of the column for the next step (regeneration). Specifically, this recovery and regeneration phase includes normally the sequence of the following steps:

(i) washing with solvent and/or buffer under conditions that the product is not released from the stationary phase (this washing may include several steps with different conditions, e.g. with varying pH, ionic strength, solvent/buffer composition, etc; indicated in the Figure with “wash”), (ii) elution with solvent and/or buffer under conditions that the product is released from the stationary phase (i.e. by a change of pH, a change of solvent or solvent composition, etc; also this step may include several steps with different conditions, indicated in the Figure with “elu”), (iii) cleaning in place (CIP) using an appropriate solvent and/or buffer to release everything from the stationary phase, optionally followed by sanitisation (again these steps may include several phases with several different conditions, e.g. with varying pH, ionic strength, solvent/buffer composition, etc) (iv) equilibration, typically by using the solvent and/or buffer under conditions similar or the same as in the subsequent process step, where again several steps with different conditions can be used to bring the column into equilibrium as efficiently as possible (indicated in the Figure with “equi”).

During this product recovery and regeneration phase of column 2 the flow rate can be increased compared to the flow rate of the columns in the interconnected phase. This is possible up to a factor of approximately 2 since the columns are operated in disconnected mode, which reduces the chromatographic bed height and thereby the back-pressure by approximately a factor of 2 compared to the interconnected phase. The flow rate increase in the recovery and regeneration step is generally not critical to the quality of separation, while for an optimum distribution in column 1 the loading flow rate in that column needs to be maintained at a well-defined level or profile as described above.

What is also possible in view of the above is that the recovery and regeneration of column 2 includes a preloading with feed. In the context of this application therefore the regeneration phase of column 2 may also include, after at least one of the steps of cleaning, and/or equilibration, a final step of feeding with feed so as to preload column 2. This is possible since the steps of washing, elution, cleaning, and/or equilibration, as mentioned above, can be carried out at a high flow rate and rather quickly, and in order to avoid idle time of column 2 and in order to have an as high as possible throughput, preloading of column 2 may be included into this regeneration step. Therefore the term regeneration of the column, in the context of this invention, may also include a terminal step of preloading of the corresponding column with feed. This holds true for the first switch as well as for the second switch, so in the second switch column 1 can be preloaded in the batch step in the regeneration phase.

During the subsequent IC phase of the first switch, column 1 and column 2 are loaded in series, with column 1 in the upstream position and column 2 in the downstream position.

During the subsequent B phase of the second switch, column 2 is loaded while column 1 is eluted (product recovery) and regenerated.

During the IC phase of the second switch, column 2 and column 1 are loaded in series, with column 2 in the upstream position and column 1 in the downstream position.

The cycle is repeated multiple times, which is indicated by the visual encapsulation of the flow sheets of one cycle with dashed lines in FIG. 1. In the shutdown phase, following the last cycle, both columns are eluted as in the B phase for final product recovery. Detectors are located at the outlet of each column, one detector D1 at the outlet of column 1 and one detector D2 at the outlet of column 2. The detectors can e.g. be UV detectors.

A cycle of the chromatographic process is defined as the sequence of tasks that each of the columns needs to fulfill before reaching its starting task again. Thus, a complete cycle of the twin column process comprises two switches, each switch comprising a disconnected phase and an interconnected phase.

Cyclic steady state is defined as a state in which the average amount of material withdrawn through the product outlet is constant from cycle to cycle. Typically a cyclically continuous process needs to be operated over a number of cycles (2-10 cycles) before reaching cyclic steady state.

In addition to the cyclic phase the process may include additional phases that improve the process performance in terms of throughput and product output. The additional phases include

-   -   A startup phase, which is only carried out once as the process         is started wherein the columns are interconnected as in the         interconnected phase but a larger amount of feed solution is         loaded so that the process reaches cyclic steady state faster.         The larger amount of feed compensates for the columns being not         preloaded and devoid of product at process start. When using a         startup phase, the twin column sequential loading process is in         cyclic steady state in the 2nd cycle.     -   A shutdown phase wherein the two columns are disconnected, no         new feed is loaded and the columns perform the remaining tasks         of a chromatographic cycle (i.e. typically the above steps         (i)-(iv)) including the elution in parallel or sequentially in         order to recover the entire product that is present in the         columns     -   Washing phases wherein the columns are interconnected and         washing solution is flushed through both columns in order to         adsorb the product that is present in the interstitial liquid of         the upstream column which is the next one to be eluted.

The process is preferably operated with a lower flow rate in the disconnected than in the interconnected state in order to improve the dynamic binding capacity and to obtain high capacity utilization. Generally the duration of the disconnected state is relatively large, preventing the use of the loading flow rate of the interconnected state also in the disconnected state, since breakthrough in the disconnected state would be likely to occur. When applied to continuous upstream production, the different feed flow rates of the interconnected and disconnected phases of the twin column sequential loading process require a connection of the process to a balancing container matched to the average feed flow rate.

Generally speaking therefore according to the first aspect of the present invention it relates to a purification method for the isolation of a desired product fraction from a mixture using only 2 chromatographic columns, wherein the method comprises, within one cycle to be carried out at least once, if needed several times, preferably essentially continuously, the following steps:

a first batch step B1, wherein during a batch timespan t_(B) said columns are disconnected and a first column is loaded with feed via its inlet using a first flow rate Q_(feed,B) and its outlet is directed to waste, and from a second column thereof desired product is recovered via its outlet and subsequently the second column is regenerated (this is the situation in the continuous process, if this step is carried out for the first time, the second column is either idle or preceding the first step B1 an interconnected start-up step is carried out in which the first and/or the second column are loaded with feed, as e.g. illustrated in FIG. 1 on top of the page).

As mentioned above, during this product recovery and regeneration phase with the second column the flow rate can be increased compared to the parallel operating first column. This is possible up to a factor of 2, so the flow rate in the second column during this phase can be twice as high as the flow rate in the interconnected phase. What is also possible in view of the above is that the recovery of second column includes a preloading with feed. In the context of this application therefore the regeneration phase of the second column may also include, after at least one of the steps of cleaning, equilibration, a final step of loading with feed so as to preload the second column. As mentioned above therefore the term “regeneration” of the column, in the context of this invention, may also include a terminal step of preloading of the corresponding column with feed. This holds true for the first switch as well as for the second switch.

As concerns the loading of the column, as mentioned above, the flow rate can be kept constant or, according to a preferred embodiment, it is possible to start with a high flow rate and according to a desired profile to reduce the flow rate towards the end of the loading of the first column in this step. Or it is possible, in a first phase of this loading of the first column, to have a high flow rate, and in a second final phase, a lower flow rate (step function).

This is followed by a first interconnected step IC1, wherein the outlet of the first column is connected to the inlet of the second column during an interconnected timespan t_(IC),

wherein the first column is loaded beyond its dynamic breakthrough capacity with feed via its inlet using a second flow rate Q_(feed,IC) which is the same or larger than the first flow rate Q_(feed,B), and the outlet of the second column is directed to waste.

In this interconnected step it is possible to have a subsequent washing step in order to make sure that in the upstream column solvent and/or buffer carrying non-adsorbed product is not washed out to waste in the initial phase of the subsequent batch step but is washed and adsorbed either in the remaining free capacity of the upstream column or of the downstream column. Correspondingly therefore during a subsequent washing timespan (t_(wash,IC)) which is larger or equal to 0 s in this interconnected step it is possible to carry out washing such that the outlet of the first column is connected to the inlet of the second column, the first column is loaded with solvent and/or buffer which is free from feed material, and the outlet of the second column is directed to waste.

This first batch step B1 and interconnected step IC1 are followed by essentially analogous steps but with the positions of the columns exchanged, i.e. the first column of the above first batch step and first interconnected step switches position to become the second column, and the second column of the above first batch step and first interconnected step switches position to become the first column. In other words, the above first batch step and first interconnected step are followed by a second batch step B2 analogous to the first batch step B1 but with exchanged columns, such that the first column 1 of the first batch step B1 is the second column 1 of the second batch step B2, and the second column of the first batch step B1 is the first column of the second batch step B2. Then this is followed by a second interconnected step IC2, analogous to the first interconnected step IC1 but with exchanged columns, such that the upstream column of the first interconnected step IC1 is the downstream column of the second interconnected step IC2, and the downstream column of the first interconnected step IC1 is the upstream column of the second interconnected step IC2.

The batch timespan t_(B) is, according to a preferred embodiment, chosen to be the accumulated time required for the recovery and regeneration of the respective column. Preferentially, this accumulated time is given by the accumulated time required for the steps of: (i) washing with solvent and/or buffer under conditions that the product is not released from the stationary phase; (ii) elution with solvent under conditions that the product is released from the stationary phase (e.g. by running a gradient, this step may include outlet fractionation); (iii) cleaning in place using a solvent and/or buffer to release everything from the stationary phase; (iv) equilibration, preferably by using the solvent and/or buffer under conditions similar or the same as in the subsequent process steps. It was found that normally the rate limiting element of the batch step is actually the recovery and regeneration of the product from the respective column. This is what takes a rather long time. It was further unexpectedly found out that an enormous increase in throughput can be obtained if during the batch step the flow rate of the other column which is not subject to recovery and regeneration, i.e. of the column which is being loaded with feed, is adapted to be as low as possible but just high enough to preload the column as much as possible within the timeframe available. According to a first preferred embodiment therefore in the interconnected step IC (i.e. in the first and the second interconnected step) the second flow rate Q_(feed,IC) is adapted to be larger than the first flow rate Q_(feed,B). Preferably the second flow rate Q_(feed,IC) is at least 10% larger, more preferably at least 25% larger, most preferably 1.5-2.5 times larger than the first flow rate Q_(feed,B). As will be detailed further below, the values are adapted by setting the second flow rate to an as high as possible value for the interconnected situation, and by choosing an appropriately optimised low value for the first flow rate, which is typically in the range of 50-90% of the second flow rate.

As pointed out above, one key element of the proposed process is the fact that the capacity utilization of the upstream column in the interconnected phase is maximized by loading the upstream column beyond its breakthrough point (the breakthrough point corresponds to the point where product starts to elute from the upstream column during loading) so that the downstream column takes up already a certain fraction of product and is preloaded. To be more specifically in this respect, and to achieve this running of the interconnected phase beyond the breakthrough point, the parameters can, according to a preferred embodiment, be adapted as follows: in the interconnected step IC the second flow rate Q_(feed,IC) and/or the interconnected timespan t_(IC) can be chosen such that at the end of interconnected timespan t_(IC) the feed concentration at the outlet of the upstream column is in the range of 30-90% (breakthrough value X) of the feed concentration at the inlet of the upstream column. Depending on the profile this condition can however be a problem in a situation where by taking this criterion product already elutes at the outlet of the downstream column. Therefore the above should preferably be taken with the proviso that the values of the second flow rate Q_(feed,IC) and/or the interconnected timespan t_(IC) are adapted such that at the end of the interconnected timespan t_(IC) at the outlet of the downstream column the product concentration is below a breakthrough value of 0.25-5%, preferably of 1-2.5%, and that the elution volume value corresponding to this breakthrough value is preferably multiplied by a safety factor in the range of 60-90%. The target is to have an as high as possible value of Q_(feed,IC), which is possible by taking the smaller of EV_(1H,X) and EV_(Y), where X is the desired breakthrough value of the upstream column, see further details given below.

According to yet another preferred embodiment, the disconnected phase may comprise a simple cleaning of one of the columns and a more thorough cleaning of the columns may be carried out in prolonged disconnected phases regularly every m cycles, wherein m is larger or equal to two.

According to yet another preferred embodiment, the columns are affinity chromatography material loaded columns, wherein the chromatographic stationary phase can be in the form of particles, such as beads, and/or membranes and/or monoliths.

For the control and monitoring of the corresponding process, but also for setting it up as will all be further detailed below, at the outlet of each column a detector for the analysis of the components at the outlet can be located, wherein preferably both detectors are of the same type. This can be any kind of detector suitable to detect the desired product, and it should be adapted such that at least for the desired product under the chosen operating conditions it is not reaching detection saturation, such that quantitative detection is possible. These detectors can e.g. be detectors selected from one or a combination of the following detectors: UV detector, visible light detector, IR detector, fluorescence detector, light scattering detector, refractive index detector, pH detector or conductivity detector.

As pointed out above, these cycles, in case of continuous feed, can be carried out continuously and repeatedly until e.g. exchange of column material is necessary or until the feed flow is interrupted necessitating a stop.

However for setting up the process, it is, according to a preferred embodiment, advisable to start with a startup step preceding the first cycle, in which the columns are interconnected and wherein a larger amount of feed solution is loaded onto the upstream column in comparison with an interconnected step IC of a cycle of the method. Indeed in such a start-up step the upstream column is not, as in continuous operation, already preloaded due to a preceding batch step, so in this start-up step the upstream column has a higher effective capacity which can then be filled up to enter the first cycle. The corresponding time is adapted and set up is detailed further below.

If the process has to be stopped, according to yet another preferred embodiment the last cycle can be followed by a shutdown step, in which the two columns are disconnected, and both columns are subjected to product recovery and column regeneration.

Preferentially, the desired product is one or a group of antibodies, antibody fragments, fusion proteins, recombinant glycoproteins, and/or plasma proteins or combination thereof. The process generally speaking thus operates in a cyclic manner wherein one cycle comprises the following alternating phases:

-   -   A first batch phase B1 wherein the columns are disconnected and         the column that has been the downstream column in the preceding         interconnected phase is continued to be loaded using a first         feed flow rate, which is the same or lower than a second feed         flow rate in an interconnected step, and wherein the column that         has been the upstream column in the preceding interconnected         phase performs the tasks of a typical chromatographic cycle that         follow the feeding step (such as washing, elution, cleaning,         re-equilibration);     -   A first interconnected phase IC1 wherein first feed solution and         optionally thereafter wash solution is loaded onto the preloaded         column and the columns are interconnected so that the stream         exiting the preloaded upstream column enters the downstream         column     -   A second batch phase B2 wherein the columns are disconnected and         the column that has been the downstream column in the preceding         interconnected phase IC1 is continued to be loaded, at a lower         flow rate than in the interconnected step, and wherein the         column that has been the upstream column in the preceding         interconnected phase IC1 performs the tasks of a typical         chromatographic cycle that follow the feeding step (such as         washing, elution, cleaning, re-equilibration), and wherein the         column that is continued to be loaded is positioned upstream in         the subsequent interconnected phase IC2;     -   A second interconnected phase IC2 wherein the columns are         interconnected in the opposite order as in the preceding         interconnected step IC1 and first feed solution and optionally         thereafter wash solution is loaded onto one of the columns and         the columns are interconnected so that the stream exiting the         upstream column enters the downstream column.

Setup Method:

For the setting up of the process as outlined above the duration t and the flow rate Q have to be determined for each of the batch B and the interconnected IC step.

As pointed out above, the rate determining steps within the batch step are generally the recovery and regeneration steps of the column which is not loaded with feed. The time required for these steps will determine the duration t_(B) of the disconnected state. As also pointed out above, during the time available in the batch step the column which is loaded with feed should be supplied with an as low as possible feed rate for optimum capacity utilization taking however also into account that at the end of the disconnected step the corresponding column should be filled up with product optimally, so just to the extent that there is no or essentially no breakthrough of the product. To determine the corresponding feed flow rate Q_(feed,B) it is however not sufficient to just determine the breakthrough curve of the single column since this would not take into account the fact that this column has already been preloaded in the preceding interconnected step when it was the downstream column. Therefore the batch step feed rate needs to take into account to which extent the column has already been filled up with feed in the preceding interconnected step.

On the other hand in the interconnected step, for efficiency reasons, the feed rate Q_(feed,IC) should be as high as possible, and is typically chosen to be at the upper end of the feed rates specified for the corresponding columns in interconnected state. The challenging task in the determination of the parameters for the interconnected state is therefore not the flow rate but the duration t_(IC) of this step. The duration should be chosen such that the capacity of the upstream column is filled up beyond its breakthrough point to the desired extent (typically expressed as a concentration of desired product at the outlet of the upstream column relative to the feed concentration at the inlet, this ratio is normally chosen in the range of 30-90%), but at the same time it must be made sure, in particular in case of a shallow breakthrough profile, that at the moment when reaching this breakthrough of the upstream column no product is already eluting at the outlet of the downstream column.

Therefore the setting up of an optimum parameterization of the process is far from trivial and in the following a protocol shall be given for finding such parameterization in a systematic manner.

More specifically therefore, the invention also relates to a method for setting up a chromatographic method as outlined above, wherein

the batch step duration t_(B) of the batch step B is set to be the accumulated time required for recovery and regeneration of the respective column, and the batch step feed flow rate Q_(feed,B) applied to the respective column in the batch step B is set such that at the end of the batch step duration t_(B) at the outlet of the column there is essentially no desired product elution, taking into account that in the preceding step this column has already been preloaded. The interconnected step feed flow rate Q_(feed,IC) on the other hand is set to a desired value and the interconnected step duration t_(IC) is set such that at the end of the interconnected step duration t_(IC) at the outlet of the upstream column the desired product concentration is in desired range, preferably in the range of 30-90%, of the concentration at its inlet, with the proviso that there is essentially no desired product elution at the outlet of the downstream column.

For the determination of the batch step feed flow rate Q_(feed,B) and the interconnected step duration t_(IC) preferably at least one breakthrough curve (or a series of breakthrough curves) of a single column using the interconnected step feed flow rate Q_(feed,IC) is recorded (e.g. using fractionation and analysis) and used. If needed, in particular in case of very shallow breakthrough profiles with the risk of product elution at the outlet of the downstream column in the interconnected step, further at least one breakthrough curve of interconnected columns using the interconnected step feed flow rate Q_(feed,IC) can be recorded and used. Furthermore at least one breakthrough curve (or a series of breakthrough curves) of a single column using a lower batch step feed flow rate Q_(feed,B) than the interconnected step feed flow rate Q_(feed,IC) is recorded and used for the determination of the parameters, wherein preferably the lower batch step feed flow rate Q_(feed,B) for the determination of this breakthrough curve is chosen to be 50-90% of the interconnected step feed flow rate Q_(feed,IC).

For the twin column process, generally speaking, the following operating parameters have to be determined prior to running the process:

t_(IC) (interconnected state duration), i_(IC,wash) (interconnected state wash duration, optional), t_(B) (disconnected state duration), t_(startup) (startup step duration, optional), Q_(feed,IC) (interconnected phase feed flow rate), Q_(feed,B) (disconnected phase feed flow rate), Q_(feed,startup) (startup state feed flow rate, optional), disconnected state elution parameters, and final elution parameters.

The disconnected state elution parameters relate to single column chromatography and include the number, duration and flow rates of the washing, elution, stripping, cleaning, sanitization, and re-equilibration steps that are applied to the loaded column in order to recover the product and to prepare the column for the uptake of new product. The disconnected state elution parameters are determined usually by a screening approach based on the purity requirements and/or on individual standard guidelines in the chromatographers institution and are therefore not a part of the parameter determination procedure described in the following. The total duration of the disconnected state procedure is referred to as t_(B) in the following.

The same holds true for the final elution parameters of the sequential loading column process where product is recovered from the two columns individually (see FIG. 1).

The startup step is optional, but it is advantageous since it shortens the time the process requires to reach steady state.

For the determination of the operating parameters t_(IC) and t_(startup) the recording of breakthrough curves is required which preferably are fractionated and analyzed by offline analysis. However, if the evaluation of the breakthrough curves is carried out based on the chromatograms only and not on the offline fraction analyses, in addition a calibration of the breakthrough signals with the feed signal recorded by the respective detector has to be carried out, preferably determined in the absence of the columns.

In summary, the design method comprises the following elements:

-   -   experimentally determining an elution volume EV₂, corresponding         to a breakthrough value multiplied with a safety factor for two         sequentially interconnected columns at the desired maximum         loading flow rate,     -   running a breakthrough curve for a single column at the desired         maximum flow rate and determining the elution volume EV_(1H,1),         corresponding to a low breakthrough value,     -   determining the elution volume EV_(1H,X), from the single column         breakthrough curve at high flow, corresponding to a high         breakthrough value X for a single column at the desired maximum         flow rate (X is preferably 30-90%),     -   running a second breakthrough curve for a single column at the         flow rate significantly lower than the desired maximum flow rate         and determining the elution volume EV_(1L,1), corresponding to a         low breakthrough value,     -   determining the value EV_(Y), which is equivalent to the smaller         one of the two values EV_(1H,X) and EV₂,     -   determining a preload value PL that corresponds to the amount of         product present in the downstream column when the feed volume of         EV_(Y) has been loaded onto the two columns in series. This         value is computed from the breakthrough curve of a single column         by means of integration with EV_(Y) as upper boundary,     -   determining a target load value TL that corresponds to the         amount of product that can be loaded additionally onto the         preloaded column in the disconnected phase B. This value is         computed from EV_(1L,1) and PL,     -   determining the interconnected state switch time t_(IC) using         EV_(1L,1) and EV_(Y). The calculation takes into account that         the upstream column is preloaded when entering the         interconnected state and that the downstream column needs to be         continued to be loaded with feed in the subsequent disconnected         state,     -   optionally determining the startup time t_(startup) from t_(IC)         and EV_(1L,1),     -   selecting Q_(feed,IC) and Q_(feed,startup) the same as the flow         rate used to record the breakthrough curve of the two         interconnected columns,     -   determining the duration of the disconnected phase B, t_(B), by         summing up the durations of the times required for the different         steps of washing, elution, cleaning, equilibration,     -   calculating the feed flow rate of the disconnected state using         the values of TL and t_(B), comparing it to the low flow rate         used to determine the second breakthrough curve, and selecting         the smaller value as Q_(feed,B).     -   determining the duration and flow rate of the wash step         t_(wash,IC) that follows the loading step in the interconnected         state using the values Q_(feed,IC) and V_(dead).

More details on the calculations are laid down in the preferred embodiments further below. A software can be used to automatically carry out the setup steps described above and in the following.

The measurement range of the detector should be adapted once before starting the method in order to enable the detector to measure at least the elution peak area and preferably the breakthrough area in the linear range of the detector (this not only applies to the setup but also to the control as further outlined below). For instance for a UV detector used in protein chromatography, if the determination of absorption at 280 nm wavelength is in the non-linear range of the detector, the wavelength may be changed to e.g. 300 nm wavelength which is most likely in the linear range of the detector for the typical protein concentrations encountered in protein chromatography.

The design method is also applicable in case of a large impurity signal where the detectors are in the non-linear range. In this case offline analyses must be used to evaluate the breakthrough curves.

The initial operating parameters of the twin-column sequential countercurrent loading chromatography process, t_(IC), t_(B), t_(startup), t_(wash,IC), Q_(feed,IC), Q_(wash,IC), Q_(feed,B), Q_(feed,startup) that may serve also as starting point for control and optimization methods are, now in more detail, determined by the following procedure:

(a) Calculation of the duration of the disconnected state t_(B) by summing up of the durations of the single steps carried out with the column which is not fed with feed following the protocol of the chromatographic cycle including typically washing, elution, cleaning, optionally sanitization, and re-equilibration steps (see also above). (b) Determination of the elution volume breakthrough value EV₂ of two interconnected columns using the desired maximum feed flow rate Q_(feed,IC) of the interconnected state. The flow rates Q_(feed,IC) and Q_(feed,startup), respectively, correspond to this maximum flow rate, and this is usually determined using a high-end value and provided for by the specification of the producer of the column material or equipment. More precisely this step (b) comprises the determination of the elution volume EV corresponding to a certain breakthrough value for instance 1% breakthrough up to 5% breakthrough with respect to the feed concentration, and multiplying this elution volume EV with a safety factor Z between 60% and 90% (both dependent on the specific conditions and targets, e.g. using values that are also used for single column chromatography). The breakthrough elution volume value, multiplied by Z, is designated EV₂. EV₂ (index 2 for two interconnected columns) is determined by evaluation of the dynamic breakthrough curve of two sequential columns (DBC₂), i.e. by using the chromatogram or by fractionation of the flow-through during load and by offline fraction analysis. Basically this measurement is carried out to find out at which elution volume in the interconnected state product starts to elute at the outlet of the downstream column. This is to make sure that the interconnected step duration is not chosen longer than what is possible in view of this breakthrough elution profile. (c) Determination of the elution volume breakthrough value EV_(1H,1) (first index 1 stands for single column, H stands for high flow rate, and second index 1 for 1% breakthrough threshold) of a single column using the previously chosen feed flow rate Q_(feed,IC), and recording the full breakthrough curve DBC₁ (dynamic breakthrough curve with column). The elution volume corresponding to a certain low breakthrough value of the single column, preferably 1% breakthrough with respect to the feed concentration is determined from the full breakthrough curve. This low breakthrough value elution volume of the single column is designated EV_(1H,1). The breakthrough value is determined by evaluation of the chromatogram or by fractionation of the flow through during load and by offline fraction analysis. The determination of EV_(1H,1) is not mandatory, however for the purpose of limited integration in step (f) it is advantageous to have this value available. (d) Determination, from the breakthrough curve determined in step (c), of the X % breakthrough elution volume EV_(1H,X) corresponding to a high load of the single column. X is typically a number between 30 and 90%. This elution volume value is designated EV_(1H,X). The aim of this is to determine the moment at which the interconnected phase needs to be terminated. However the value determined here as it stands does not take into account that the upstream column has already been preloaded in the preceding step, and it also does not take into account that it has to be prevented that product elutes at the outlet of the downstream column during the interconnected phase. For preventing the latter the condition in step (e) is applied. (e) Determining the smaller one of the two value EV₂ and EV_(1H,X). This value is designated EV_(Y). The purpose of this condition is to make sure that no product elutes at the outlet of the downstream column during the interconnected phase. (f) Determination of the preload value PL, calculated by integrating the difference of the calibrated breakthrough curve DBC₁ of the single column (as determined in above (c)) and the calibrated breakthrough curve DBC₂ of the interconnected columns (as determined in above (b)) with the upper boundary of EV_(Y), divided by the column volume of the single column according to equation 1:

$\begin{matrix} {{{PL} = {{\frac{1}{V_{col}} \cdot {\int_{{EV}_{{1\; H},1}}^{{EV}_{Y}}{{DBC}_{1\; H}({EV})}}} - {{{DBC}_{2}({EV})}\ {{EV}}}}}{{EV}_{Y} = {\min \left\{ {{EV}_{{1H},X};{EV}_{2}} \right\}}}} & \left( {{equation}\mspace{14mu} 1} \right) \end{matrix}$

It is also possible to use zero as lower starting point for the integration.

In this step in other words the product mass per column volume is calculated by which the downstream column is already preloaded due to what happens in the interconnected step, which in turn is necessary for determining how much capacity is left for loading in the batch step.

(g) Determination of a second breakthrough value EV_(1L,1) of a single column using a lower flow rate Q_(feed,BT1L) than for the previous single column breakthrough determination. Typically Q_(feed,BT1L)=W %·Q_(feed,IC), with W=50-90%: Recording the full breakthrough curve DBC_(1L) at least until EV_(Y) and determination of the elution volume corresponding to a certain low breakthrough value, preferably 1% breakthrough with respect to the feed concentration (the same % value as in the determination of the single column breakthrough value at the higher flow rate can be chosen). This elution volume is designated EV_(1L,1), wherein the breakthrough value is determined from DBC_(1L) by evaluation of the chromatogram or by fractionation of the flow through during load and by offline fraction analysis. This value, indicating by how much the column is already charged with desired product at the end of the batch step, now allows the determination of the possible duration of the interconnected step in (h). (h) Determination of the interconnected state switch t_(IC) time by subtracting Z %·EV_(1L,1) from EV_(Y) and dividing the complete term by the feed flow rate Q_(feed,IC) in the interconnected state (i.e. the feed flow rate that was used to record the breakthrough curves DBC₂ of the two sequential columns in step (b)), according to equation 2:

t _(IC)=(EV_(Y) −Z %·EV_(1L,1))/Q _(feed,IC)  (equation 2)

(i) Determination of the target load value TL, from the preload value PL and the breakthrough curve DBC_(1L) of the single columns by subtracting the dead volume from the elution volume corresponding to 1% breakthrough of the value EV_(1L,1), multiplied with the safety factor Z, and multiplying the difference with the feed concentration value, dividing said product by the column volume and subtracting PL from this value, according to equation 3:

TL=((Z %·EV_(1L,1) −V _(dead))·c _(feed))/V _(col)−PL  (equation 3)

This value thus represents the actually available capacity of the column during the batch step feed loading, i.e. the available capacity taking the preload during the interconnected step into account. This value now allows the determination of the feed flow rate in the batch state in the following step (j).

(j) Determination of the feed flow rate of the disconnected state, Q_(feed,B), according to equation 4 by multiplying TL with the column volume, dividing by the feed concentration, and dividing by the duration of the disconnected state, t_(B), which is determined by the protocol for the chromatographic cycle which comprises washing, elution, cleaning and re-equilibration steps, and optionally further steps of sanitization.

Q _(feed,B)=(TL·V _(col))/(c _(feed) ·t _(B))  (equation 4)

If the flow rate Q_(feed,B) determined by this calculation is larger than the flow rate that was used to record the single column breakthrough curve at the lower flow rate, the latter flow rate should be used as Q_(feed,B), i.e. Q_(feed,B)=Q_(feed,BT1L).

(k) Optional determination of the startup time t_(startup) by dividing the difference of Z %·EV_(1L,1) and V_(dead) by the feed flow rate Q_(feed,IC) and adding this value to t_(IC), according to equation 5. The flow rate during startup is the same as the feed flow rate in the interconnected phase.

An interconnected state startup step is optional but it can be used to reach the cyclic steady state faster.

t _(startup) =t _(IC)+(Z %·EV_(1L,1) −V _(dead))/Q _(feed,IC)=(EV_(Y) −V _(dead))/Q _(feed,IC)  (equation 5)

(l) Determination of the duration of the interconnected state washing step t_(wash,IC) that follows the interconnected phase loading step by dividing the dead volume by the flow rate Q_(wash,IC) which is of the same value as Q_(feed,IC) but uses washing buffer instead of feed (equation 6):

t _(wash,IC) =k·V _(dead) /Q _(wash,IC)  (equation 6)

, with k as rational number≧1.

In the above equations, the dead volume V_(dead) corresponds to the elution volume of a non-adsorbing component from the point of feed to the point of detection (including one column). It can be determined either by tracer experiments, or by evaluating the impurity signals recorded by the two UV detectors in the sequential loading of two columns

The above method can be used also to determine the operating parameters for a k-column sequential loading process with k>2 with the difference that the disconnected state tasks that typically include washing, elution, cleaning, sanitization, and re-equilibration steps are distributed among more than one phase (as in the case of the twin column process).

Control, Monitoring and Optimization Method:

The invention further, and also independently of the above two column process and of the corresponding setup procedure, relates to a method to be used for chromatographic sequential loading process monitoring with detector comparison. The control and monitoring method requires there to be at least 2 columns which are operated, alternatingly, in interconnected and disconnected state, and switch positions after such a sequence of interconnected and disconnected state. Furthermore the control and monitoring method requires that a detector is located downstream of each column capable of detecting the desired product when it passes the detector. Preferably the detector should be adapted to be operating in a quantitative regime during all phases of the process, i.e. when faced with undesired impurities but also when faced with desired product.

The gist of the control and monitoring method is to compensate for differences in the at least two detectors present by using the repetitious cycle of interconnected and disconnected state and to exploit the fact that the two columns and therefore also the two detectors alternatingly take over the same function. By comparing the results of the detectors associated with the columns at situations where they should actually show identical results due to the identical function of the associated column, control is possible in particular by calculation of the corresponding ratio between the two detector results in this situation and checking whether the same ratio is true for different positions in the process where also the same ratio should result.

It should be noted in this respect that so far control and monitoring of these processes has only taken place in the past by checking either whether there is any product at the outlet during product elution (simple yes/no detection but not quantitative) or by comparing the inlet with the outlet of single columns using two detectors at these positions, which however inherently poses the problem of calibration of these two detectors. All this is avoided by the proposed simple and straightforward process.

The monitoring and control method for a chromatographic multicolumn sequential loading process with at least two columns preferably comprises at least the steps of determining online at least the elution peak signal ‘A_(peaki)’ (measured by detector i) of a single cycle, corresponding to the product of interest, recorded by detectors located at the outlet of each column, evaluating the signal by means of suitable algorithms and deriving control actions therefrom.

The detector signals should be quantitatively representative of product mass and/or concentration.

The nomenclature used in the following method descriptions and preferred embodiments, relates, without loss of generality to the areas measured by UV detectors, and is explained in the following:

The peak area A_(peaki) is defined as the area confined by the elution peak curve and a horizontal baseline corresponding to the value of the equilibrated empty column (see FIG. 2 and FIG. 5) or a baseline defined by other criteria such as points of operating parameter changes (e.g. end of the washing step, start of the cleaning step).

In FIG. 2, the impurity signal plateau value is indicated by dotted horizontal lines. The signal plateau corresponds to the non-adsorbing impurities. The breakthrough areas A_(ICiU), A_(ICiD), and A_(Bi) are confined by the breakthrough curve and the horizontal baseline and are indicated by two arrows each. “B” and “IC” indicate disconnected and interconnected phases, respectively. In FIG. 2, the area above the plateau level in the 1st B phase B1 is designated “A_(B1)”. “A_(B1)” corresponds to a product loss from column 1 during the loading step in the disconnected phase. The area above the plateau level in the 1st IC phase IC1 is designated A_(IC1U) (“IC” stands for “interconnected phase”, “1” stands for the number of the column/detector, “U” stands for upstream). The area A_(IC1U) corresponds to product leaving the upstream column during the interconnected phase, however it does not correspond to product leaving the system since the product enters the second column. The product elution peak in the 2nd B phase is designated “A_(peak1)” and the area above the plateau level in the 2nd IC phase is referred to as A_(IC1D) (“D” stands for downstream). In the 2nd IC phase, column 1 is downstream and receives the product eluting from the upstream column 2. The area A_(IC1D) is zero in the presented case corresponding to zero loss from the column 1 when column 1 is in the downstream position in the IC2 phase, indicating that the entire product eluting from the upstream column 2 is adsorbed in column 1;

The method distinguishes between the breakthrough areas of the downstream columns in the interconnected loading phase of the columns, A_(ICiD) (measured by detector i for column i), which reflect a loss of product; the breakthrough areas of the columns that are loaded in the disconnected state, A_(Bi), which also reflect a loss of product, and the breakthrough areas of the upstream columns in the interconnected loading phase of the columns, A_(ICiU). As said, the latter area represents the material that is transferred from the upstream to the downstream column and does not reflect a product loss.

When comparing A_(ICiD), A_(ICiU) and A_(Bi) and A_(peaki), the potentially different flow rates during the recording of the areas need to be taken into account. Areas can be normalized for different flows by multiplication with the flow rates.

The monitoring and control method is based on the comparison of at least the peak areas A_(peaki), among different cycles and/or among different detectors as described further below and in the preferred embodiments. The method can be also used in the case of high impurity signals where A_(ICiD), A_(ICiU) and A_(Bi) cannot be measured. The method is capable of monitoring column capacity and feed concentration changes in a cycle-to-cycle manner, and to derive control actions from the measured areas.

In long term process operation, different causes such as decreasing feed concentration and simultaneous column degradation may lead to the same effects on breakthrough and peak areas causing the same control actions with the load as only parameter. It is an advantage of the presented method that it does not require detailed knowledge on the cause of the effects for successful process control. The aim of the control actions is generally to maintain a constant yield or a constant load in order to ensure a constant product concentration. Possible control actions for adapting the load in the subsequent chromatography cycles or cycle phases include, but are not limited to, changes of the feed flow rate in the B and/or IC phases, load durations, durations of the phases B and/or IC or changes of feed concentrations. The yield is adapted by changing the load, too. For instance, in order to obtain a higher yield, the load must be lowered.

Another embodiment of the invention relates to an optimization method to maximize load and throughput of the process. This approach is relevant in process development in order to maximize the productivity of the process and to appropriately size the equipment when scaling up the process.

The optimization method includes the comparison of at least the eluate peak signal and/or breakthrough signals from different cycles of the process, the cycles to be evaluated can be consecutive but can also be spaced apart, to derive control actions there from. With regard to FIG. 2, the optimization would aim at maximizing A_(peaki) while keeping A_(Bi) to a minimum in the following cycles. A_(ICiD) is automatically minimized if A_(Bi) is minimized

As in the case of process monitoring and control, possible control or optimization actions for adapting the load in the subsequent chromatography cycles or cycle phases include, but are not limited to, changes of the feed flow rate in the B and/or IC phases, load durations, durations of the phases B and/or IC or changes of feed concentrations.

In order to maximize the process performance the load is increased step-wise in a cycle-to-cycle fashion. The newly recorded areas, following the step change are compared to the areas of previous cycle and the process performance is re-calculated using the new values. In the case that measurements of breakthrough areas and peak elution area are possible due to a favorable detector signal, the process performance can be improved based on the areas determinations of a single cycle. In case only the peak elution areas can be determined (due to a large impurity signal), additional data from earlier or later cycles of the same run are required to determine the optimization potential of the process and to carry out the optimization. Further optimization method details are laid down in the preferred embodiments.

The method can be advantageously used to determine suitable operating parameters by calculation, avoiding a time-consuming experimental determination procedure.

By determining elution peak areas and breakthrough areas from the downstream column in the interconnected state and the loading column in the disconnected state, product loss and yield can be quantified online A feed signal is not required.

The tedious task of detector calibration is also not required since the control actions are determined either from signals recorded with the same detector or from signals recorded from different detectors that have been normalized through calculation of ratios between signals recorded by each of the detectors. Another major advantage over prior art descriptions is that by using the peak areas as basis for process control and optimization, the method can be applied for the control of processes for the purification of products from feedstocks that cause a very large impurity signal that is in the non-linear range of the detector and therefore do not allow measurement of breakthrough signals.

The described control and optimization methods can be used also for all other sequential loading chromatography processes with more than two columns since the concept of sequential loading always entails the presence of an area A_(ICiD), corresponding to a product loss from the most downstream column. The number of areas A_(ICiU), measured by inline detectors, and corresponding to product that is transferred from an upstream into a downstream column, may vary as more columns are interconnected, but these areas do not reflect product losses (see FIG. 2). The areas A_(peaki) correspond to product recovery from a single column in all common multicolumn sequential loading processes. Areas A_(Bi) are present if the multicolumn process comprises steps where one or more of the columns are loaded while being disconnected from the other columns Some multicolumn processes comprise such steps while others do not.

For all processes, the common aim of control is to keep A_(peaki), A_(ICiD), and A_(Bi), possibly also A_(ICiU), within certain specification limits. The common aim of optimization is the maximization of A_(peaki) while keeping A_(ICiD), and, if present, also the areas A_(Bi), to a minimum.

More specifically, in the case that A_(ICiU), and A_(Bi) are accessible to quantitative measurement by the detectors due to a low impurity signal, the proposed method of monitoring and/or control and/or optimization of the process comprises the following elements:

(a) Measuring the disconnected phase signals A_(peaki) corresponding to the effluents of all columns (i) in a product elution position in a disconnected state in at least one cycle or parts of one cycle using the detectors at the columns outlets; (b) Measuring the interconnected phase signals A_(ICiU) corresponding to the effluents of all columns (i) in an interconnected state upstream and/or downstream positions in at least one cycle or parts of one cycle using the detectors at the columns outlets; (c) Comparing the disconnected phase signals A_(peaki) among the two detectors, preferably by calculating the ratios between disconnected phase signals A_(peaki) of the different detectors; (d) Comparing the interconnected phase signals A_(ICiU) among the two detectors, preferably by calculating the ratios between interconnected phase signals A_(ICiU) of the different detectors; (e) Comparing the values of the disconnected phase signals A_(peaki) comparison in step (c) and the interconnected phase signals A_(ICiU) comparison (d) among two detectors in order to quantify the degree of the signal magnitude difference, and using this ratio and/or the ratios of (c) and/or (d) for control and/or monitoring. Specifically, a control action can be initiated based on this difference, e.g. actions to be implemented for at least one future cycle of the chromatographic process can be based on this difference, the chromatographic process can be halted due to e.g. deterioration of the columns and/or insufficient feed or the like, and/or it can be used to optimize the parameters of the process such as step timing, flow rates, load, yield (recovery), throughput, buffer consumption of the process.

In particular in the case that A_(ICiU), and A_(Bi) are accessible to quantitative measurement by the detectors due to a low impurity signal and the product concentration in the feed is constant, which is the case in a large number of applications, it is, according to another process of monitoring and/or control and/or optimization, possible to use a reduced process which comprises the following elements:

(a) Measuring the interconnected phase signals A_(ICiU) corresponding to the effluents of at least two, preferably all columns (i) in an interconnected state upstream and/or downstream positions in at least one cycle or parts of one cycle using the detectors at the columns outlets; (b) Comparing the interconnected phase signals A_(ICiU) among the at least two, preferably all detectors, preferably by calculating the ratios between interconnected phase signals A_(ICiU) of the different detectors and using this ratio for control and/or monitoring. Specifically, a control action can be initiated based on this difference/ratio, e.g. actions to be implemented for at least one future cycle of the chromatographic process can be based on this difference, the chromatographic process can be halted due to e.g. deterioration of the columns and/or insufficient feed or the like, it can be used to optimize the parameters such as timing, flow rates, load, yield (recovery), throughput, buffer consumption of the process.

In particular in the case that A_(ICiU) and A_(Bi) are not accessible to quantitative measurement by the detectors due to a high impurity signal, and the feed concentration is constant, which is the case in a large number of applications, the proposed process of monitoring and/or control and/or optimization to maximize load and throughput of the process comprises the following elements:

(a) Measuring the disconnected phase signals A_(peaki) corresponding to the effluents of all columns in a product elution position in a disconnected state in at least one cycle or parts of one cycle using the detectors at the columns outlets; (b) Comparing the disconnected phase signals A_(peaki) among the two detectors, preferably by calculating the ratios between disconnected phase signals A_(peaki) of the different detectors; (c) comparing the values of the disconnected phase signals A_(peaki) or ratios thereof of at least one cycle among two detectors in order to quantify the degree of the signal magnitude difference, and using this difference for control and/or monitoring. Specifically, a control action can be initiated based on this difference, e.g. actions to be implemented for at least one future cycle of the chromatographic process can be based on this difference, the chromatographic process can be halted due to e.g. deterioration of the columns and/or insufficient feed or the like, it can used to optimize the parameters such as timing, flow rates, load, yield (recovery), throughput, buffer consumption of the process.

In the abovementioned control methods, preferably the control action is the change of the column load by reduction or increase of the feed flow rate in one or multiple phases of the cyclic process. In another preferred embodiment, the control action is the change of the duration of the interconnected or the disconnected phase, respectively.

In other embodiments the control actions include the reduction or increase of the feed interval duration, changes of the feed composition (for instance by dilution).

Further embodiments of the invention are laid down in the dependent claims.

BRIEF DESCRIPTION OF THE DRAWINGS

Preferred embodiments of the invention are described in the following with reference to the drawings, which are for the purpose of illustrating the present preferred embodiments of the invention and not for the purpose of limiting the same. In the drawings,

FIG. 1 shows a schematic representation of a twin column countercurrent sequential loading process, where the process comprises an optional startup phase, a cyclic phase and a shutdown phase;

FIG. 2 shows an example of a UV signal recorded at the outlet of column 1 by detector UV1 (black solid line) for the steps in FIG. 1. The plateau value of the non-adsorbing impurities is indicated by horizontal dotted lines;

FIG. 3 shows breakthrough curves, wherein diamonds indicate the single column breakthrough curve at high flow rate, squares indicate the single column breakthrough curve at low flow rate, triangles indicate the breakthrough curve from two serially interconnected columns; the elution volumes EV_(1H,1), EV_(1L,1), EV_(1H,75), and EV₂ are indicated by solid vertical lines; the feed concentration is indicated by a dashed horizontal line;

FIG. 4 shows the performance parameters yield, productivity, IgG concentration, and buffer consumption (average values) for the five cycles of the sequential countercurrent loading process of example 2;

FIG. 5 shows signals recorded during cycles 2, 3, 4 by the two UV detectors (UV1, UV2) positioned at the column outlets of a twin-column sequential loading chromatography process, wherein the interconnected phases are denoted with “IC”, the disconnected phases are denoted with “B”; the phase borders are represented by black dashed short vertical lines; the cycle borders are represented by bold dashed long vertical lines; the areas corresponding to the breakthrough from the upstream column are marked with A_(IC2U,n) for UV2 (column 2 is upstream) and A_(IC1U,n) for UV1 (column 1 is upstream), respectively, where n stands for the cycle number; for instance A_(IC1U,3) refers to the area corresponding to the breakthrough from column 1 in the 3rd cycle with column 1 being in the upstream position; the peak areas corresponding to the eluates from the columns that have been in the upstream position in the previous interconnected phases are marked with A_(peak2,n) for UV2 (column 2 was upstream in the previous interconnected phase) and A_(peak1,n) for UV1 (column 1 was upstream in the previous interconnected phase), respectively, where n stands for the cycle number; for instance A_(peak1,3) refers to the area corresponding to the product elution from column 1 in the 3rd cycle; the baselines used for the area determinations are indicated with thin dashed lines;

FIG. 6 shows the signals recorded during cycles 2 and 3 by the two UV detectors (UV1, UV2) positioned at the column outlets of a twin-column sequential loading chromatography process; the phase borders are represented by dashed short vertical lines; the cycle borders are represented by dashed long vertical lines; the areas corresponding to the breakthrough of the non-adsorbing impurities cannot be determined due to non-linearity of the detector signal; the peak areas corresponding to the product eluates from the columns that have been in the upstream position in the previous interconnected phases are marked with A_(peaki2,n) for UV2 (column 2 was upstream in the previous interconnected phase) and A_(peaki,n) for UV1 (column 1 was upstream in the previous interconnected phase), where n stands for the cycle number 2 or 3; for instance A_(peak2,3) refers to the area corresponding to the product elution from column 2 in the 3rd cycle; the baselines used for the area determinations are indicated with thin dashed lines;

FIG. 7 shows an overlay of signals from two runs of a twin-column sequential loading chromatography process recorded by the UV detector UV2 during one cycle (B1, IC1, B2, IC2); for the sake of simplicity the cycle index n is omitted; the signal of the first run (UV2-I, full line) corresponds to operating parameters with a load of 48 g/L, the signal of the second run (UV2-II, dotted line) corresponds to operating parameters with a load of 30 g/L (decreased feed flow rate in the disconnected state); the interconnected phases are denoted with “IC”, the disconnected phases are denoted with “B”; the phase borders are represented by black dashed vertical lines. The plateau value of the non-adsorbing impurities is indicated by a horizontal dashed-dotted line. The areas referred to in the example 8 description are indicated by arrows;

FIG. 8 shows the productivity (diamonds) and yield determined by the optimization method described in example 9 (full circles) and by offline HPLC analyses (empty circles) as a function of the load; and

FIG. 9 shows the peak area values A_(peak) from UV1 (diamonds) and UV2 (squares) according to example 11 as a function of the load; the values corresponding to the three lowest loads (UV1) and the two lowest loads (UV2), respectively, were fitted using linear functions (equations displayed in figure); in addition, the yield determined by the optimization method described in example 11 (full circles) and by offline HPLC analyses (empty circles) is shown as a function of the load.

DESCRIPTION OF PREFERRED EMBODIMENTS Example 1 Initial Determination of Operating Parameters for a Twin Column Countercurrent Sequential Loading Process

The initial operating parameters for a twin-column countercurrent sequential loading process for the purification of an IgG from clarified cell culture harvest using protein A affinity chromatography were determined based on the procedure outlined above. The breakthrough curves were recorded, fractionated and analyzed by offline Protein A analysis using a Poros A/20 column (Life technologies, USA) to determine the IgG concentrations. The concentration of IgG in the feed was 1.0 g/L. The columns were of 0.5 cm inner diameter and 5.0 cm length. A protocol for the elution and regeneration of a loaded column was developed including a wash step of 6 min, an elution step of 7 min, a cleaning step of 6 min, a first equilibration step of 3 min, and a second equilibration step of 3 min; all at a flow rate of 1 mL/min Thus, the total duration of the elution and regeneration steps was t_(B)=25 min

A first breakthrough curve for a single column was recorded for a feed flow rate of 1 mL/min, a second breakthrough curve for a single column at a feed flow rate of 0.5 mL/min and a third breakthrough curve at a feed flow rate of 1 mL/min for two columns in series. With the load factor X=75%, and the safety factor Z=90%, the following elution volume values were obtained (see above descriptions and equations):

EV_(1H,1)=19.9 mL, EV_(1L,1)=23.4 mL, EV_(1H,75)=48.4 mL and EV₂=60.8 mL.

An overlay of the offline analysis results together with marks for the elution volume values is provided in FIG. 3. Since EV_(1H,75)<EV₂, EV_(Y) was set equal to EV_(1H,75). The preload value PL was computed by integration approximation using a trapezoid rule to be PL=11.2 g/L and the target load value was determined to be TL=16.3 g/L. The feed flow rate in the disconnected state was determined to be 0.53 mL/min. Since this flow rate was larger than the flow rate used to determine the second breakthrough curve, the latter value was selected as feed flow rate, Q_(feed,B)=0.50 mL/min. The duration of the interconnected phase of the sequential loading process was computed to be t_(IC)=25 min and the duration of the startup phase was t_(startup)=47.5 min. The feed flow rate in both cases was Q_(feed,startup)=Q_(feed,IC)=1 mL/min. The interconnected washing step duration was t_(wash,IC)=1 min and the flow rate was Q_(wash,IC).

Example 2 Operation of a Twin Column Countercurrent Sequential Loading Process

A twin column countercurrent sequential loading process was used for the capture of an IgG monoclonal antibody from clarified cell culture harvest using a Protein A affinity stationary phase packed into two columns of 0.5 cm inner diameter and 5.0 cm length.

The process was operated with the following parameters on Contichrom Lab-10 equipment from ChromaCon AG, Switzerland. The UV detection wavelength was 305 nm. The process was run using the operating parameters determined in example 1, which are summarized in table 1.

TABLE 1 Operating parameters for twin-column countercurrent sequential loading process of example 2 including the startup phase. Q_(feed) indicates the feed flow rate, Q_(buffer) indicates the flow rate of all other steps; t is the duration of the substeps of the disconnected phase B and the interconnected phase IC, respectively. The parameters for the final elution correspond to the B parameters except for the feed flow rate, which is zero during the final elution step. Q_(feed) Phase step Q_(buffer) buffer [mL/ t [—] [—] [mL/min] [—] min] [min] B wash 1.0 25 mM Phosphate, pH 0.5 6.0 7.0 elute 1.0 25 mM Citrate, pH 3.0 0.5 6.0 clean 1.0 0.1M NaOH 0.5 7.0 equilibrate 1 1.0 25 mM Citrate, pH 3.0 0.5 3.0 equilibrate 2 1.0 25 mM Phosphate, pH 0.5 3.0 7.0 IC feed 0 — 1.0 25.0 wash 1.0 25 mM Phosphate, pH 0.0 1.0 7.0 startup feed 0 — 1.0 47.5

The operating parameters of the chromatographic process (such as feed flow rate and switch times) were not changed throughout the run. The feed concentration was artificially increased by approximately 0.01-0.02 g/L every cycle (see table 2) to simulate a subtle change in the upstream process.

After the initial startup phase the purification process was run continuously over five cycles before being eluted in the final elution phase. The effluents corresponding to the feed, the product fraction, the feed flow through fraction and the cleaning fraction were analyzed by offline protein A HPLC analysis in order to determine the IgG concentrations. From the volume of the fractions and the product concentrations, the product masses and performance parameters were computed.

The yield was calculated by comparing the product mass in the product fraction with the product mass in the feed fraction. The productivity was calculated by division of the product mass (obtained in one cycle) by the cycle duration and the total column volume (i.e. the volume of the two columns together). The buffer consumption was calculated by dividing the overall volume of buffers used within one cycle by the product mass in the product fraction of that cycle. The feed concentration and the performance parameters are summarized in table 2 and the evolution over time is shown in FIG. 4. The example shows that high yield and productivity values can be obtained using the described process despite subtle changes of the feed concentration.

TABLE 2 Feed concentration, IgG concentration, yield, productivity and buffer consumption of the sequential countercurrent loading process as average values for the five cycles of the process. buffer feed conc pool conc yield productivity consumption Cycle [g/L] [g/L] [%] [g/L/h] [L/g] 1 1.01 5.10 53.8 21.0 1.1 2 1.03 5.24 93.3 21.5 1.1 3 1.04 5.28 92.6 21.7 1.1 4 1.06 5.37 92.6 22.1 1.0 5 1.08 5.49 93.1 22.6 1.0

Example 3 Detector Comparison, Low Impurity Signals

A twin column countercurrent sequential loading process was operated with the following parameters on Contichrom Lab-10 equipment from ChromaCon AG, Switzerland. Two detector cells giving different signals for the same sample were used for demonstration. The operating parameters are summarized in Table 3.

TABLE 3 Operating parameters for twin-column countercurrent sequential loading process of example 3. Q_(feed) indicates the feed flow rate, Q_(buffer) indicates the flow rate of all other steps; t is the duration of the substeps of the disconnected phase B and the interconnected phase IC, respectively. The parameters for the final elution correspond to the B parameters except for the feed flow rate, which is zero during the final elution step. The UV detection wavelength was 300 nm. Q_(buffer) Q_(feed) Phase step [mL/ buffer [mL/ t [—] [—] min] [—] min] [min] B wash 1.0 25 mM Phosphate, pH 7.0 0.2 6.0 elute 1.0 25 mM Citrate, pH 3.0 0.2 6.0 clean 1.0 0.1M NaOH 0.2 7.0 equilibrate 1.0 25 mM Citrate, pH 3.0 0.2 3.0 1 equilibrate 1.0 25 mM Phosphate, pH 7.0 0.2 3.0 2 IC feed 0 — 1.0 24.5 wash 1.0 25 mM Phosphate, pH 7.0 0.0 1.0 startup feed 0 — 1.0 45.0

The chromatograms from the UV detectors UV1 and UV2 located at the outlet of each column, recorded during cycles 2, 3 and 4 are shown in FIG. 5. The process was in cyclic steady state in cycles 3 and 4.

During the interconnected phases, the UV detectors record a signal that corresponds to the load flow-through of the upstream and the downstream column, respectively. The chromatograms of the process in cyclic steady state are explained referring to cycle 2 starting at 100 min.

In the following, the index n is used to describe the n-th cycle of the process. Thus, for instance, the area A_(peak2,3) corresponds to the elution peak area recorded for column 2 (by detector 2) in the 3^(rd) cycle.

In the disconnected phase B the product was recovered from column 2. The disconnected phase typically includes at least one washing step prior to elution, and typically a strip and/or clean step followed by at least one re-equilibration step. The product peak is indicated by A_(peak2,2) (recorded by UV2). In parallel to the washing, elution, cleaning and re-equilibration of column 2, column 1 was loaded with feed at lower flow rate as the feed flow rate in the interconnected state. The signal UV1 corresponded to the non-adsorbing impurities that flowed through. The signal reached a constant plateau, indicating that no product was breaking through during the B phase.

In the subsequent interconnected phase IC, the columns were interconnected and column 1 was placed in the upstream position and continued to be loaded. The rising signal (UV1) recorded at the outlet of column 1 at the end of the interconnected phase indicated that product was breaking through (A_(IC1U,2)). However, this product was not lost but re-adsorbed in the downstream column 2. While breakthrough was detected at column 1 (UV1), no breakthrough was detected at column 2 (UV2) indicating that the entire product was re-adsorbed. At the end of the interconnected phase B column 1 was washed with fresh buffer such that the product present in the liquid volume of the upstream column 1 was adsorbed in the downstream column 2.

In the following disconnected phase B the product was recovered from column 1 using the same protocol of washing, elution, strip and/or clean, re-equilibration steps that was used in the previous disconnected phase for column 2. The product peak is indicated by A_(peak1,2) (recorded by UV1). At the same time column 2 was loaded with feed at a lower flow rate as the feed flow rate in the interconnected state. The signal UV2 corresponded to the impurities that flowed through. Again, the signal reached a constant plateau, indicating that no product was breaking through.

In the subsequent interconnected phase IC, column 2 was continued to be loaded and the rising signal (UV2) at the end of the interconnected phase indicated that product was breaking through from column 2 (A_(IC2U,2)). However, this product was not lost but re-adsorbed in the downstream column. While breakthrough was detected from column 2 (UV2), no breakthrough was detected at column 1 (UV1) indicating that the entire product was re-adsorbed. At the end of the interconnected phase B column 2 was washed with fresh buffer such that the product present in the liquid volume of column 2 was adsorbed in the downstream column 1.

After the interconnected phase had been completed, a new cycle was started.

In the process, before being washed and eluted in the disconnected phase, the respective columns are in the upstream positions in the interconnected phase and loaded such that product is breaking through into the downstream column where it is re-adsorbed (see example 2). The breakthrough in the interconnected phase is proportional to the areas designated with A_(IC2U,n) (UV2, column 2) and A_(IC1U,n) (for UV1, column 1) in FIG. 5, with n as cycle number.

In FIG. 5 furthermore the peak areas corresponding to the elution of the columns in the disconnected phase that have previously been in the upstream position in the interconnected phase are shown and designated A_(peak1,n) (for UV1, column 1) and A_(peak2,n) (UV2, column 2), with n as cycle number. The baselines for the peak areas were drawn from the elution peak start to the UV signal points that corresponded to the end of the elution step in the disconnected state. Since the UV signal was not at zero at this point, the baselines are not horizontal. However, in order to obtain comparable results for A_(peak) it is more important to draw the baseline in a consistent manner for each peak in each cycle rather than drawing a horizontal baseline.

The determined areas are listed in Table 1.

In the ideal case of absolutely identical columns and detectors A_(IC1U,n) (table 4, column 1) would be equal to A_(IC2U,n) (table 4, column 3) and A_(peak1,n) (table 4, column 2) would be equal to A_(peak2,n) (table 3, column 4). In practice both columns and detectors are significantly different, thus in most cases the areas A_(IC1U) and A_(IC2U) are significantly different and the areas A_(peak1) and A_(peak2) are significantly different from each other.

This is confirmed by the ratio A_(IC2U)/A_(IC1U) (table 4, column 5) calculated for every single cycle. The ratio shows that A_(IC2U) in cyclic steady state (cycles 3 and 4) is about 1.3 times larger (30%) than A_(IC1U). In the case of different areas it is very important to determine if the difference is due to a detector with different properties, e.g. amplification, or if it is due to a column with different, potentially deteriorated capacity. Making a judgment based on the different areas alone may lead to the erroneous replacement of a column that actually had an acceptable capacity.

By comparison of the ratios A_(peak2)/A_(peak1) (table 4, column 6) it becomes clear that also A_(peak2) is about 1.3 times larger (30%) than A_(peak1). Also the ratio of the sums of the breakthrough and the peak areas (A_(IC2U,n)+A_(peak2,n))/(A_(IC1U,n)+A_(peak1,n)) is about 1.3 (table 3, column 10).

Together, this information shows that the detector signal of UV2 is in general 1.3-fold larger than the detector signal of UV1.

In case the ratios A_(IC2U)/A_(IC1U) and A_(peak2)/A_(peak1) would have been different, it would have indicated that the columns differ in capacity. In that case the ratio of the sums of the breakthrough and the peak areas (table 3, column 10) would have been used to determine the difference of the detector signals.

TABLE 4 Areas and ratios derived from FIG. 5, given in [mAU min]. The columns 5-10 marked with “ratio” represent ratios of values listed in other columns. For instance the ratio 3/1 (column 5) stands for a division of the values of column 3 by the values of column 1 (thus A_(peak2)/A_(peak1)) 1 2 3 4 5 6 7 8 9 10 measured measured measured measured ratio ratio ratio ratio ratio ratio cycle A_(IC1U) A_(peak1) A_(IC2U) A_(peak2) 3/1 4/2 2/1 4/3 7/8 (3 + 4)/(1 + 2) 2 128 1157 189 1500 1.48 1.30 9.0 7.9 1.14 1.31 3 111 1112 149 1419 1.34 1.28 10.0 9.5 1.05 1.28 4 113 1112 146 1419 1.29 1.28 9.8 9.7 1.01 1.28

Example 4 Column Capacity Monitoring, Low Impurity Signals

The twin column countercurrent sequential loading process of example 3 was evaluated for column capacity changes. The feed was not changed during the run and the feed concentration was constant. Thus only the column capacity was potentially changing, for instance due to fouling or harsh cleaning. The explanations refer to the nomenclature used in FIG. 5.

The fact that A_(ICiU,n)=A_(ICiU,n-1) and/or A_(peaki,n)=A_(peaki,n-1) for both columns (see example 2) indicates the column capacity has stayed constant from one cycle to the other.

If A_(ICiU,n)>A_(ICiU,n-1) and/or A_(peaki,n)<A_(peaki,n-1) would have been measured with significant difference it would have been indicative of a decreasing column capacity. Typically the accuracy of the area determination is 1%, thus an increase of A_(ICiU,n) by 1% over A_(ICiU,n-1) or a decrease of A_(peaki,n) by 1% over A_(peaki,n-1) cannot be attributed to a loss of capacity.

The method may be also used to evaluate the column performance of cycles that are not successive.

Example 5 Column Capacity Monitoring, High Impurity Signals

A twin column countercurrent sequential loading process was operated for the capture of a product from feed material with a large impurity content. The feed material was the same throughout the entire run (constant product concentration in feed). The operating parameters are summarized in table 5.

TABLE 5 Operating parameters for twin-column countercurrent sequential loading process of example 5. Q_(feed) indicates the feed flow rate, Q_(buffer) indicates the flow rate of all other steps; t is the duration of the substeps of the disconnected phase B and the interconnected phase IC, respectively. The parameters for the final elution correspond to the B parameters except for the feed flow rate, which is zero during the final elution step. The UV detection wavelength was 305 nm. Q_(buffer) Q_(feed) Phase step [mL/ buffer [mL/ t [—] [—] min] [—] min] [min] B wash 1.0 25 mM Phosphate, pH 7.0 0.2 6.0 elute 1.0 25 mM Citrate, pH 3.0 0.2 9.0 clean 1.0 0.1M NaOH 0.2 7.0 equilibrate 1.0 25 mM Citrate, pH 3.0 0.2 2.0 1 equilibrate 1.0 25 mM Phosphate, pH 7.0 0.2 4.0 2 IC feed 0 — 1.0 24.5 wash 1.0 25 mM Phosphate, pH 7.0 0.0 1.0 startup feed 0 — 1.0 45.0

Due to the large impurity content of the feed material the detectors were in the non-linear range of detection (over-saturated), as shown in FIG. 6, and it was not possible to determine the areas corresponding to the product break-through in any of the phases of the process (A_(ICiU), A_(Bi)).

However, based only on the peak areas A_(peaki,n) it was possible to determine if the column capacity had deteriorated from one cycle to the other. The product elution peak areas for the 2nd and the 3rd cycle of the process were determined to be A_(peak2,2)=756 mAU min, A_(peak2,3)=764 mAU min, A_(peak1,2)=805 mAU min, A_(peak1,3)=809 mAU min By calculating the ratios A_(peak2,3)/A_(peak2, 3) and A_(peak1,3)/A_(peak1,2), respectively, it becomes obvious that the areas are identical with a difference of 1% which corresponds to the accuracy of the area determination. This means that the column capacities are identical with a difference of maximum 1%. This is expected since typically the capacity does not change dramatically within two subsequent cycles. The method may be also used to evaluate the columns performance of cycles that are not successive.

It has to be noted that in the case of large impurity signals it is not possible to decide based on only A_(peaki) data if the capacities of the two columns are different or if the detectors are give different signals for the same product concentrations. However, if A_(peaki) measurements are carried out with a constant feed concentration it can be tested by changing the load if the load is in the linear range of product adsorption. It is assumed that no dramatic column capacity decrease takes place within A_(peaki) measurements of two cycles. If the load is in the linear range of product adsorption and the comparison is made between at least two peak areas A_(peaki) from each detector the detectors signal difference can be determined by forming the average of the ratios of A_(peak2,n)/A_(peak1,n). The detectors signal difference determination becomes more accurate if the comparison A_(peak2,n)/A_(peak1,n) is made for more cycles.

More information on the linear range of product adsorption is provided in example 10.

Example 6 Feed Concentration and Column Capacity Monitoring, Low Impurity Signals

The twin column countercurrent sequential loading process of example 3 was evaluated for the effect of product concentration changes in the feed material and simultaneous column capacity changes.

The operating parameters of the chromatographic process (such as feed flow rate and switch times) were not changed over the cycles but the feed concentration is assumed to be variable and the column capacity is assumed to be constant or to decrease. The different scenarios for possible column degradation and potential feed concentration changes are summarized in table 5.

In example 3 the breakthrough areas are the same A_(ICiU,n)=A_(ICiU,n-1) and the peak areas are the same A_(peaki,n)=A_(peaki,n-1) for both columns in two consecutive cycles (3 and 4), indicating that neither the feed quality nor the column performance have significantly decreased from one cycle to the other.

In the following, more examples of relative behavior of A_(ICiU) and A_(peaki) and the possible causes with respect to feed concentration and column capacity are provided.

If A_(ICiU,n)<A_(ICiU,n-1) and A_(peaki,n)<A_(peaki,n-1) is detected the feed concentration must have decreased. The column capacity may have stayed the same from one cycle to the other but may have also decreased. An increasing ratio A_(peaki,n)/A_(ICiU,n) from one cycle to the other is indicative of the column quality staying constant or that the capacity is decreasing to a lesser extent than the column load. A decreasing ratio A_(peaki,n)/A_(ICiU,n) from one cycle to the other is indicative of the column quality staying constant or the capacity decreasing to a stronger extent than the column load.

If A_(ICiU,n)>A_(ICiU,n-1) and A_(peaki,n)≧A_(peaki,n-1) are measured the feed concentration must have increased from one cycle to the other. The column capacity may have stayed the same from one cycle to the other but may have also decreased. An increasing ratio A_(peaki,n)/A_(ICiU,n) from one cycle to the other is indicative of the column quality staying constant or that the capacity is decreasing to a lesser extent than the column load increases. A decreasing ratio A_(peaki,n)/A_(ICiU,n) from one cycle to the other is indicative of the column quality staying constant or the capacity decreasing to a stronger extent than the column load.

If A_(ICiU,n)>A_(ICiU,n-1) and A_(peaki,n)<A_(peaki,n-1) are measured the column capacity must have decreased. The feed concentration must have changed such that the load was increased to a stronger extent than the capacity of the columns decreased. Theoretically, the capacity of the columns may decrease to the same extent like the increase of the load such that the effects cancel out. In that case it is recommended to calculate the ratio of the areas corresponding to the total product that is eluting for two different cycles, (A_(peaki,n)+A_(ICiU,n))/(A_(peaki,n-1)+A_(ICiU,n-1)), for the same detector. If the ratio is >1, the feed concentration must have increased from one cycle to the other.

TABLE 5 Changes in column capacity and feed concentration from cycle n − 1 to n and the effects on the areas A_(ICiU) and A_(peaki) that are determined from the chromatograms. Column capacity changes include: capacity decrease “↓”, constant capacity “=”; Product concentration changes in the feed include the following: increase “↑”, decrease in “↓”, no change “=”. columns feed A_(ICiU) A_(peaki) = = A_(ICiU,n) = A_(ICiU,n−1) A_(peaki,n) = A_(peaki,n−1) =/↓ ↓ A_(ICiU,n) < A_(ICiU,n−1) A_(peaki,n) < A_(peaki,n−1) = ↑ A_(ICiU,n) > A_(ICiU,n−1) A_(peaki,n) ≧ A_(peaki,n−1) ↓ = A_(ICiU,n) > A_(ICiU,n−1) A_(peaki,n) < A_(peaki,n−1) ↓ ↑ A_(ICiU,n) > A_(ICiU,n−1) effect may cancel out, check (A_(peaki,n) + A_(ICiU,n))/(A_(peaki,n−1) + A_(ICiU,n−1))

Example 7 Feed Concentration and Column Capacity Monitoring, High Impurity Signals

The twin column countercurrent sequential loading process of example 5 was evaluated for the effect of product concentration changes in the feed material and simultaneous column capacity changes. In the following, the product concentration in the feed will abbreviated with “feed concentration”

The operating parameters of the chromatographic process (such as feed flow rate and switch times) were not changed over the cycles but the feed concentration was assumed to be variable and the column capacity is assumed to be constant or to decrease.

Due to the large impurity content of the feed material the detectors were in the non-linear range of detection (over-saturated), as shown in FIG. 6, and it was not possible to determine the areas corresponding to the product break-through in any of the phases of the process (A_(ICiU), A_(Bi)).

The fact that A_(peaki,n)=A_(peaki,n-1) for both columns indicates that a.) neither the feed concentration nor the column performance have decreased significantly or b.) that the column capacity for both or one of the columns has decreased to the same extent as the feed concentration has increased from cycle n−1 to cycle n.

If A_(peaki,n)>A_(peaki,n-1) is detected for both or one of the columns indicates that the feed concentration has increased from cycle n−1 to n and the column capacity has stayed equal or decreased to a lesser extent as the feed concentration has increased, for both or one of the columns.

If A_(peaki,n)<A_(peaki,n-1) is detected for both or one of the columns, either the feed concentration has decreased from cycle n−1 to n or the column capacity has decreased or both the feed concentration and the column capacity have decreased. Possibly also the feed concentration has increased from cycle n−1 to n but the column capacity has decreased to a stronger extent.

Example 8 Process Control, Low Impurity Signals

Two twin column countercurrent sequential loading processes for the capture of a product from feed material with a large impurity content were evaluated for the effect of product concentration changes in the feed material and simultaneous column capacity changes. In the following, the product concentration in the feed is abbreviated with “feed concentration”. The twin-column countercurrent sequential loading process conditions are listed in table 7.

TABLE 7 Operating parameters for the twin-column countercurrent sequential loading processes of example 8. Q_(feed) indicates the feed flow rate, Q_(buffer) indicates the flow rate of all other steps; t is the duration of the substeps of the disconnected phase B and the interconnected phase IC, respectively. Q_(feed) was 0.82 mL/min and 0.19 mL/min, respectively. The parameters for the final elution correspond to the B parameters except for the feed flow rate, which is zero during the final elution step. The UV detection wavelength was 300 nm. Q_(buffer) Q_(feed) Phase step [mL/ buffer [mL/ t [—] [—] min] [—] min] [min] B wash 1.0 25 mM Phosphate, 0.82/0.19 6.0 pH 7.0 elute 1.0 25 mM Citrate, 0.82/0.19 9.0 pH 3.0 clean 1.0 0.1M NaOH 0.82/0.19 7.0 equilibrate 1 1.0 25 mM Citrate, 0.82/0.19 2.0 pH 3.0 equilibrate 2 1.0 25 mM Phosphate, 0.82/0.19 4.0 pH 7.0 IC feed 0 — 1.0 24.5 wash 1.0 25 mM Phosphate 0.0 1.0 pH 7.0 startup feed 0 — 1.0 45.0

Based on the areas A_(ICiU), A_(Bi) and A_(peaki), control actions can be derived to maintain certain levels of A_(peaki), which correspond to the concentration of the product of interest in the product pool.

As outlined in example 6, A_(ICiU) and A_(peaki) experience certain trends in response to feed concentration and column capacity changes. The aim of process control is to maintain A_(peaki) within certain defined limits. The possible control actions include a change of the load and are listed in detail above.

The control actions based on the trends of the areas A_(ICiU) and A_(peaki) are summarized in the following:

-   -   If the feed concentration remains constant and the column         quality remains constant no control action is required.     -   If the feed concentration decreases and the column capacity         remains the same or decreases the load may be increased to         increase A_(peaki) (see constraint reported below).     -   In case the feed concentration is increasing and the column         quality remains the same or decreases to a lesser extent, the         load must be reduced to maintain the desired level of A_(peaki).     -   In case the feed concentration stays equal and the column         capacity decreases the load may be increased to increase         A_(peaki) (see constraint reported below).     -   In case the feed concentration is increasing and the column         capacity is decreasing to a larger extent, the load may be         increased to maintain the desired level of A_(peaki), taking         into account the constraint explained below.

The above cases show that the effects of column capacity deterioration and feed concentration change in the periodic countercurrent loading process can be controlled by changing only the load. Summarizing the above control actions, if A_(peaki) decreases beyond the specified limits the load should be increased (see constraint below) and if A_(peaki) increases beyond the specified limit the load should be reduced.

The constraint for load increases is given by the dynamic binding capacity. The more the capacities of the downstream column in the interconnected state and of the column that is loaded in the disconnected state are exceeded, the more product is lost and the lower the final recovery of the process.

In order to avoid product losses, the breakthrough of product from the column that is loaded during the disconnected state and the breakthrough of product from the downstream column in the interconnected state must be minimized

In process chromatograms these constraints imply that the signals from the columns that are loaded during the disconnected state and the signals from the downstream columns in the interconnected state must not exceed the plateau value that corresponds to the level of the impurities which are not adsorbed. This constraint is graphically illustrated in FIG. 7 for chromatograms of the runs with two different loads (run I: 48 g/L, run II: 30 g/L, only the signal of detector UV2 is shown).

FIG. 7 shows that the signals from the downstream column in the interconnected phase IC1 (UV2-I and UV2-II) reach the plateau value that corresponds to the non-adsorbing impurities (see arrows in FIG. 7 in phase IC1, for both 30 g/L and 48 g/L load). Thus the areas A_(IC2D-I) and A_(IC2D-II) confined by the signal curve and the impurity plateau baseline are not larger than zero and no product is lost in either of the runs during the interconnected state from the downstream column.

In the disconnected phase B2 the area in between the breakthrough curve and the impurity plateau baseline is zero for run II (area not indicated in FIG. 7 since the signal curve and the impurity plateau baseline match exactly). Thus, in run I, no product is eluted into the waste (and lost) during the B phase. In contrast, for run I, the area indicated with A_(B2-I) is larger than zero and corresponds to product breakthrough in the loading step of the disconnected phase (i.e. product loss).

In the interconnected phase IC2 the areas in between the signal curve of the upstream column in the interconnected state and the impurity plateau baseline are designated A_(IC2U-I) and A_(IC2U-II), respectively. In phase IC2 the breakthrough from the previously loaded column either starts (30 g/L load, UV2-II) or respectively continues (48 g/L load, UV2-I), however the column is in the upstream position and the product leaving the column is completely adsorbed in the downstream column. Therefore the product that corresponds to the areas A_(IC2U-I) and A_(IC2U-II), recorded at the upstream column outlet in the interconnected phase, remains in the system and is not lost.

The area values, obtained by integration, that correspond to product losses are listed in Table 8.

In order to estimate if the product losses were significant in cyclic steady state the areas A_(ICiD) and A_(Bi) were compared with the respective elution peak areas A_(peaki) by calculating the product loss ratios PLR1=(A_(B1)+A_(IC1D))/(A_(peak1)+A_(B1)+A_(IC1D)) for column 1 (signals not shown in FIG. 7) and PLR2=(A_(B2)+A_(IC2D))/(A_(peak2)+A_(B2)+A_(IC2D)) for column 2 using the area values listed in Table 8 for each of the runs I and II. In this case, since A_(IC1D)=A_(IC2D)=0 for both runs I and II, the equations can be facilitated to PLR1=A_(B1)/(A_(peak1)+A_(B1)) and PLR2=A_(B2)/(A_(peak2)+A_(B2)). The average product loss ratio was then calculated as PLRavg=(PLR1+PLR2)/2. For the run with the 48 g/L load, it was found that PLR1 was 14.5% and PLR2 was 11.4% and the average ratio PLRavg was about 13%, indicating a significant product loss. The corresponding product yield was Y=100%-PLRavg=87%. In contrast, all product loss ratios for the 30 g/L run (run II) were zero due to A_(B1)=A_(B2)=0 and consequently the yield was 100%.

The yield values were confirmed by offline HPLC protein A analytics to be 90% (run I) and 100% (run II), which is in good agreement with the values determined by the online area evaluation. In the presented case the evaluation was carried out online by manual integration using the Contichrom evaluation software (ChromaCon AG, Zurich, Switzerland) but it can be fully automated using suitable integration algorithms and automatically trigger control actions for the subsequent cycles.

Consequently, based on the online analysis results, in run I the load would be lowered by means of a suitable control action in order to avoid product losses in future cycles. Possible control actions include but are not limited to a reduction of the feed flow rate in the disconnected state, a reduction of the feed flow rate in the interconnected state and a reduction of the interconnected state duration, as described further above.

It is worth noting that depending on the individual column capacity and detector calibration, the areas A_(B1) and A_(B2B) may be significantly different. In order to reliably estimate if potential product losses are significant and require control actions, the areas A_(B1) and A_(B2) are put into perspective with the corresponding peak areas A_(peak1) and A_(peak2), respectively, and an average product loss ratio should be estimated based on both columns/detectors. The same applies for the areas A_(IC1D) and A_(IC2D). Furthermore it is worth noting that if A_(B1) and A_(B2) are zero, also A_(IC1D) and A_(IC2D) must be zero (see run II case).

TABLE 6 Data from the cyclic steady state from two operating points of twin-column sequential loading chromatography process. Run I: 48 g/L load, run II: 30 g/L load. The determined areas correspond to the areas indicated in FIG. 7 for UV2. The areas for UV1 were evaluated too but are not shown in FIG. 7 for clarity. Column 2 shows the feed flow rate in the disconnected state of the process. 4 7 2 3 UV1 5 6 UV2 1 Q_(feed,IC) Load A_(peak1) A_(B1) A_(IC1D) A_(peak2) Run [mL/min] [g/L] [mAU * min] [mAU * min] [mAU * min] [mAU * min] I 0.82 48 344 58.3 0 379 II 0.19 30 253  0.1 0 282 10 11 12 8 8 avg product Yield Yield 1 A_(B2) A_(IC2D) loss ratio (online) (offline) Run [mAU * min] [mAU * min] [%] [%] [%] I 49 0 13.0  87.0  90.5 II  0 0  0.0 100.0 100.0

Example 9 Optimization of a Twin Column Countercurrent Sequential Loading Process, Low Impurity Signals

In order to optimize process performance it is desirable to maximize the load of the run in order to process more material in the same amount of time using the same columns.

In the previous examples suitable criteria for the identification of product losses have been explained in detail. The criteria can serve as optimization tool since they define the maximum load of the process. Based on the criteria, the load of the process may be increased just until the point where product losses start to occur.

A twin column countercurrent sequential loading process was run for the capture of an IgG monoclonal antibody from clarified cell culture harvest using a Protein A affinity stationary phase packed into two columns of 0.5 cm inner diameter and 5.0 cm length in order to demonstrate the optimization procedure.

The process was operated with the parameters listed in table 9 on Contichrom Lab-10 equipment from ChromaCon AG, Switzerland.

TABLE 9 Operating parameters for the twin-column countercurrent sequential loading process of example 9. Q_(feed) indicates the feed flow rate, Q_(buffer) indicates the flow rate of all other steps; t is the duration of the substeps of the disconnected phase B and the interconnected phase IC, respectively. Q_(feed) was increased every two cycles. The parameters for the final elution correspond to the B parameters except for the feed flow rate, which is zero during the final elution step. The UV detection wavelength was 300 nm. Q_(buffer) Q_(feed) Phase step [mL/ buffer [mL/ t [—] [—] min] [—] min] [min] B wash 1.0 25 mM Phosphate, 0.0/0.19/ 6.0 pH 7.0 0.43/0.62/ 0.82 elute 1.0 25 mM Citrate, 0.0/0.19/ 9.0 pH 3.0 0.43/0.62/ 0.82 clean 1.0 0.1M NaOH 0.0/0.19/ 7.0 0.43/0.62/ 0.82 equilibrate 1 1.0 25 mM Citrate, 0.0/0.19/ 2.0 pH 3.0 0.43/0.62/ 0.82 equilibrate 2 1.0 25 mM Phosphate, 0.0/0.19/ 4.0 pH 7.0 0.43/0.62/ 0.82 IC feed 0 — 1.0 24.5 wash 1.0 25 mM Phosphate, 0.0 1.0 pH 7.0 startup feed 0 — 1.0 45.0

The same feed material was used throughout the run. The feed flow rate in the interconnected state was kept constant throughout the run at 1.0 mL/min.

The load flow rate in the disconnected phase was increased after every two cycles in steps from 0.0 mL/min to 0.82 mL/min (see Table 10, column 1). The flow rates corresponded to loads of 26 g/L to 48 g/L (see Table 10, column 2). For column 1, the areas A_(peak1), A_(B1), A_(IC1D) were determined (see Table 10, columns 3-5) and for column 2, the areas A_(peak2), A_(B2) and A_(IC2D) were determined (see Table 10, columns 6-8) as described in example 3 (Note: for 26 g/L load the areas of UV2 were not determined).

From the determined areas from UV1 and UV2, the average product loss ratios PLRavg and the yields were calculated (see Table 10, columns 9, 10). For comparison, the yield was determined by offline HPLC analysis (see Table 10, column 11). The productivity (see Table 10, column 12) was calculated as the amount of product produced within one cycle divided by the duration of the cycle and the total column volume based on the offline analysis values.

The buffer consumption (see Table 10, column 13) was calculated as the amount of buffer consumed (in liters) per gram of product purified based on the offline analysis values.

Table 10 shows that with increasing load the productivity increases significantly and the buffer consumption decreases. Increasing the load from 26 g/L to 43 g/L, which corresponds to an increase of more than 65%, still allows for product recovery with more than 97% yield. A further increase of the load to 48 g/L leads to significant product losses of at least 10%, resulting in a product yield of less than 90%. In the presented case, losses of more than 5% were considered inacceptable, so of the tested conditions 43 g/L was the optimal load that had a 65% larger productivity than the base case at 26 g/L and an over 65% reduced buffer consumption. Process yield, determined by online and offline analysis and the productivity are shown as a function of the load in FIG. 8.

Summarizing, the presented example shows how the sequential loading process can be optimized by increasing the load successively and by monitoring and evaluating the areas under the signal curves corresponding to the positions of potential product loss.

The effect of a load increase control action becomes evident in the following cycle, potentially allowing for a new control action. In the presented example, the feed concentration was constant but the optimization method can also be applied if the feed concentration changes or the column capacity is different among the columns and/or the capacity of the columns is decreasing. The method can be fully automated using suitable integration algorithms and automatically trigger control actions for the subsequent cycles.

Preferably the step size for load increase for process optimization is in the range of 5-10 g/L for an IgG capture using Protein A affinity chromatography. Through successive increase of the load the process the operating space corresponding to high yield is explored. As soon as an operating point with inacceptable yield is found, the process conditions should be reverted to a previous operating point that corresponded to 100% yield. If desired, the process optimization can be continued by increasing the load again by smaller increments or a load safety margin can be left by not continuing the optimization.

Preferably the process optimization is started from a point that corresponds to an intermediate load. However, if only very little process knowledge is present an optimization starting point is defined by operating parameters corresponding to 0 g/L load in the disconnected phase.

TABLE 10 Optimization procedure data of the twin-column sequential loading chromatography process (example 9). Column 1 shows the feed flow rate in the disconnected state of the process; column 2 the load; columns 3-5 the areas A_(peak1), A_(B1), A_(IC1D), respectively, of UV1 determined in the 2nd cycle after the feed flow rate change; columns 6-8 the areas A_(peak2), A_(B2), A_(IC2D), respectively, of UV2 determined in the 2nd cycle after the feed flow rate change; column 9 the average product loss ration PLRavg, column 10 the yield determined online by the area evaluation method described in the example, column 11 the yield determined through HPLC offline analysis, column 12 the productivity and column 13 the buffer consumption based on the offline analyses. 3 4 5 6 7 1 2 UV1 UV1 UV1 UV2 UV2 Q Load A_(peak1) A_(B1) A_(IC1D) A_(peak2) A_(B2) [mL/min] [g/L] [mAU * min] [mAU * min] [mAU * min] [mAU * min] [mAU * min] 0.00 26 219 0 0 n.a. n.a. 0.19 30 253 0.1 0 282 0 0.43 37 298 1.7 0 326 2 0.62 43 330 10.5 0 359 10 0.82 48 344 58.3 0 379 49 8 9 10 11 1 UV2 avg product Yield Yield 12 13 Q A_(IC2D) loss ratio (online) (offline) Prod. B.C. [mL/min] [mAU * min] [%] [%] [%] [g/L/h] [L/g] 0.00 0 0.0 100.0 100.0 14.4 1.2 0.19 0 0.0 100.0 100.0 17.2 1.0 0.43 0 0.6 99.4 99.5 20.7 0.6 0.62 0 2.8 97.2 99.6 23.9 0.4 0.82 0 13.0 87.0 90.5 24.5 0.2

Example 10 Process Control, High Impurity Signals

The example describes the control of the twin column countercurrent sequential loading process for the capture of a product from feed material with a large impurity signal (see FIG. 6) and variable concentration of product of interest throughout the entire run.

As shown in example 5, A_(B1) and A_(IC1D) as well and A_(B2) and A_(IC2D) cannot be measured due to the large impurity signal.

However in the frequently observed case of an only slowly decreasing column capacity, and with implemented safety margins for the load it can be assumed that no product is lost during in the twin-column periodic countercurrent process. Safety margins can be determined by means of offline analysis for product concentration determination. Thus, with reference to example 9, A_(B1) and A_(IC1D) as well and A_(B2) and A_(IC2D) can be assumed to be zero.

In the described case the area A_(peaki) changes only in dependence of the load, for instance due to a change of the feed concentration.

Consequently, based on only the peak areas A_(peaki) and the comparison of their development over at least two cycles, the process performance can be monitored and control actions can be derived to maintain certain levels of A_(peaki), which correspond to the desired concentration of the product of interest in the product pool.

In case the feed concentration remains constant the peak areas A_(peaki) remain constant over the cycles (A_(peak1,n)=A_(peak1,n-1) and A_(peak2,n)=A_(peak2,n-1)) and no control action is required.

In case the feed concentration increases, the peak areas A_(peaki) increase over the cycles (A_(peak1,n)>A_(peak1,n-1) and A_(peak2,n)>A_(peak2,n-1)), and a decrease of the load is required if the peak areas have run out of specification.

In case the feed concentration decreases, the peak areas A_(peaki) decrease over the cycles (A_(peak1,n)<A_(peak1,n-1) and A_(peak2,n)<A_(peak2,n-1)) and an increase of the load is required if the peak areas have run out of specification.

The control actions may be also based on comparing areas of non-successive cycles or fractions of cycles.

Example 11 Optimization of a Twin Column Countercurrent Sequential Loading Process, High Impurity Signals

The example describes the optimization of a twin column countercurrent sequential loading process for the capture of a product from feed material with a large impurity signal (see FIG. 6) and a constant concentration of product of interest throughout the entire run. The optimization method is described based on the experimental data shown in example 9 without using the values of the areas A_(B1), A_(IC1D) and A_(B2), A_(IC2D) in order to simulate the presence of a large impurity signal as described in example 5.

However in order to optimize process performance it is desirable to maximize the load in order to process more material in the same amount of time using the same columns

A successive increase of the load inevitably leads to product losses when the column capacity is exceeded. Due to the large impurity signal product losses cannot be directly identified by the areas A_(B1), A_(IC1D) and A_(B2), A_(IC2D), respectively.

Nevertheless, process optimization based only on the peak areas A_(peaki) is possible, when evaluating the peak areas of operating points with different loads.

The starting point of the process optimization corresponded to a load where no breakthrough was present, namely to a load during the interconnected state that corresponds to a value that is well below the breakthrough value of two sequential columns and with a feed flow rate of zero during the disconnected state. The starting load value corresponded to 26 g/L (see table 11, column 2). As described in example 9 the load was now increased every two cycles and the areas A_(peaki) were determined by integrating the chromatograms (table 11, column 3 (UV1) and column 6 (UV2)). After having recorded the peak areas A_(peak1) corresponding to three loads (26 g/L, 30 g/L and 37 g/L) and two loads (A_(peak2), 30 g/L, 37 g/L, the peak area from the first load was not measured), respectively, the area values were plotted as a function of the load and the data was fitted with two straight lines (see FIG. 9). The fact that the peak areas A_(peaki) could be fitted with a straight line indicates that starting at 26 g/L additional product loaded led to a proportional increase of the peak areas A_(peaki), or in other words that all additional product that is loaded is also adsorbed and that the product losses are negligible. In the presented case, the linear correlation of A_(peak1) from 26 g/L to 37 g/L justified also the use of a linear correlation of A_(peak2), which was determined using only two points (30 g/L, 37 g/L). The obtained linear correlations between peak areas and load were used to extrapolate the expected peak areas for higher loads (table 11 column 4 (UV1) and column 7 (UV2)). Further points at increased load were recorded and the determined peak areas were compared to the expected peak areas calculated by means of the correlation. At 43 g/L and 48 g/L load the measured area was smaller than the area predicted by means of the correlation. The occurrence of a difference is expected at higher loads since the stationary capacity is limited, which is not taken into account by the linear correlation. The area difference (UV delta) between the expected and measured peak areas is shown in table 11, column 5 (UV1) and column 8 (UV2). The average product loss ratio (PLR) can now be calculated by dividing UV delta by the corresponding expected peak area for each UV and by forming the average of the values of UV1 and UV2. The yield (online, determined by the presented optimization method, see table 11 column 10) can be calculated by 100%-PLR or by taking the average of the ratio of measured and expected peak area of the two detectors. For comparison, the yields determined using offline HPLC analyses are also shown (see FIG. 9). A comparison of the two yields (online, offline) shows that the chosen optimization method based on evaluation of only the peak areas was capable of identifying loads that corresponded to negligible product losses and such loads that corresponded to larger product losses. Based on both the presented optimization method and offline analyses the operating point with 48 g/L load would have been ruled out due to inacceptable product losses (>5%) and the operating point corresponding to 43 g/L would have been chosen. As described in example 9, the 43 g/L load corresponded to a 65% larger productivity than the base case at 26 g/L load and to an over 65% reduced buffer consumption.

It has to be noted that it is not possible to decide based on only A_(peaki) data if the capacity of the two columns is different or if the detectors have a different signal magnitude. However, if A_(peaki) measurements are carried out with a constant feed concentration in the linear region of the measurement range and the comparison is made between more than two peak areas A_(peaki) from each detector, as outlined in this method, information on both detector calibration and column capacity decrease can be obtained.

Summarizing, the presented example shows how the sequential loading process can be optimized by changing the load and monitoring and evaluating only the peak areas without the need to evaluate areas under breakthrough curves. For this method information of successive cycles is required to determine if the column capacity has been exceeded. The advantage of the method is that it can also be applied in cases where the impurity signal is exceeding the measurement range of the detector. It is recommended to dynamically verify the performance data corresponding to historical operating points if the process is operated over time spans where significant column degradation is expected. For this purpose the load may be changed temporary.

Both presented optimization methods (example 9 and example 11) do not require detector calibration.

TABLE 11 Optimization procedure data of the twin-column sequential loading chromatography process (example 11). Column 1 shows the feed flow rate in the disconnected state of the process; column 2 the load; column 3 the area A_(peaki) determined by integration of the chromatogram of UV1; column 4 the area A_(peaki) as estimated from the linear correlation described in the example and in FIG. 9 for UV1; column 5 the difference between the two areas of column 4 and column 3 of UV1; column 6 the area A_(peaki) determined by integration of the chromatogram of UV2; column 7 the area A_(peaki) as estimated from the linear correlation described in the example and in FIG. 9 for UV2; column 8 the difference between the two areas of column 7 and column 6 of UV2; column 9 the average product loss ration PLRavg, column 10 the yield determined online by the area evaluation method described in the example, column 11 the yield determined through HPLC offline analysis, column 12 the productivity and column 13 the buffer consumption based on the offline analyses. 3 4 6 7 1 2 UV1 UV1 expctd 5 UV2 UV2 expctd Q Load A_(peak1) A_(peak1) UV1 delta A_(peak2) A_(peak2) [mL/min] [g/L] [mAU * min] [mAU * min] [mAU * min] [mAU * min] [mAU * min] 0.00 26 219 219 1 n.a. 251 0.19 30 253 252 −1 282 282 0.43 37 298 299 0 326 326 0.62 43 330 338 8 359 362 0.82 48 344 377 33 379 399 9 10 11 1 8 avg product Yield Yield 12 13 Q UV2 delta loss ratio (online) (offline) Prod. B.C. [mL/min] [mAU * min] [%] [%] [%] [g/L/h] [L/g] 0.00 n.a. 0.3 99.7 100.0 14.4 1.2 0.19 0 −0.2 100.0 100.0 17.2 1.0 0.43 0 0.1 99.0 99.5 20.7 0.6 0.62 3 1.5 98.5 99.6 23.9 0.4 0.82 20 6.9 93.1 90.5 24.5 0.2

LIST OF REFERENCE SIGNS B Disconnected state or phase Q_(feed,BT1L) feed flow rate for single (batch state) of the sequential column operation that is loading process significantly lower than IC Interconnected state or phase maximum desired feed flow of the sequential loading rate process EV_(1H,1) elution volume conesponding t_(B) duration of the disconnected to low breakthrough based on phase of the sequential a single column breakthrough loading process curve recorded for Q_(feed,IC) t_(IC) duration of the interconnected EV_(IL,1) elution volume conesponding phase of the sequential to low breakthrough based on loading process a single column breakthrough t_(startup) duration of the startup phase curve recorded for Q_(feed,BT1L) of the sequential loading EV_(1H,X) elution volume conesponding process to a high breakthrough based t_(wash,IC) duration of the wash phase of on a single column the sequential loading process breakthrough curve recorded Q_(feed) feed flow rate, general for Q_(feed,IC) Q_(feed,IC) second flow rate, feed flow EV₂ elution volume conesponding rate during the interconnected to low breakthrough based on phase of the sequential a sequentially interconnected loading process column breakthrough curve Q_(wash,IC) wash flow rate during the recorded for Q_(feed,IC), interconnected phase of the multiplied with the safety sequential loading process factor Z. Q_(feed,B) first flow rate, feed flow rate EV_(Y) smaller of the two values during the disconnected EV_(1H,X), EV₂ phase of the sequential X magnitude of breakthrough, loading process typically 30-90%. Q_(feed,startup) feed flow rate during the W ratio between Q_(feed,BT1L) and startup phase of the Q_(feed,IC); Typically 50-90% sequential loading process Z safety factor for loading, typically 60-90% A_(Bi) area confined by V_(dead) dead volume, volume of breakthrough curve and a interstitial liquid in one horizontal baseline column corresponding to plateau V_(col) empty column volume signal value of the non- c_(feed) feed concentration adsorbing impurities DBC_(1H)(EV) single column breakthrough measured at the outlet of curve, recorded at Q_(feed,IC), as column i by detector i during a function of the elution the disconnected phase B volume A_(ICiU) area confined by DBC₂(EV) breakthrough curve of two breakthrough curve and a interconnected columns, horizontal baseline recorded at Q_(feed,IC), as a corresponding to plateau function of the elution signal value of the non- volume adsorbing impurities dEV elution volume increment for measured at the outlet of integration column i by detector i during TL Target load value in the the interconnected phase IC disconnected phase of the when column i is in the sequential loading process upstream position. PL Preload value in the A_(ICiD) area confined by interconnected phase of the breakthrough curve and a sequential loading process horizontal baseline A_(peak) area confined by the elution corresponding to plateau peak curve and a horizontal signal value of the non- baseline corresponding to the adsorbing impurities value of the equilibrated measured at the outlet of empty column or a baseline column i by detector i during defined by other criteria such the interconnected phase IC as points of operating when column i is in the parameter changes downstream position. i column index, detector index PLR product loss ratio n cycle number 

1. A chromatographic purification method for the isolation of a desired product fraction from a mixture using two chromatographic columns, wherein the method comprises, within one cycle to be carried out at least once, the following steps: a first batch step, wherein during a batch timespan said columns are disconnected and a first column is loaded with feed via its inlet using a first flow rate and its outlet is directed to waste, and from a second column thereof desired product is recovered via its outlet and subsequently the second column is regenerated; a first interconnected step, wherein the outlet of the first column is connected to the inlet of the second column during an interconnected timespan, the first column is loaded beyond its dynamic breakthrough capacity with feed via its inlet using a second flow rate which is the same or larger than the first flow rate, and the outlet of the second column is directed to waste, and wherein during a subsequent washing timespan which is larger or equal to 0 seconds, the outlet of the first column is connected to the inlet of the second column, the first column is loaded with solvent and/or buffer which is free from feed material, and the outlet of the second column is directed to waste, a second batch step analogous to the first batch step but with exchanged column positions, such that the first column of the first batch step performs the tasks of the second column of the first batch step, and the second column of the first batch step performs the tasks of the first column of the first batch step; a second interconnected step, analogous to the first interconnected step but with exchanged column positions, such that the upstream column of the first interconnected step is the downstream column of the second interconnected step, and the downstream column of the first interconnected step is the upstream column of the second interconnected step.
 2. The chromatographic method according to claim 1, wherein the second flow rate in the interconnected step is adapted to be larger than the first flow rate.
 3. The chromatographic method according to claim 1, wherein in the interconnected step at least one of the second flow rate or the interconnected timespan are adapted such that at the end of interconnected timespan the feed concentration at the outlet of the upstream column is in the range of 30-90% of the feed concentration at the inlet of the upstream column, with the proviso that the values of the at least one of second flow rate or interconnected timespan are adapted such that at the end of the interconnected timespan at the outlet of the downstream column the feed concentration is below a breakthrough value of 0.25-5%.
 4. The chromatographic method according to claim 1, wherein the batch timespan is chosen to be the accumulated time required for the recovery and regeneration of the respective column.
 5. The chromatographic method according to claim 1, wherein the columns are affinity chromatography material loaded columns.
 6. The chromatographic method according to claim 1, wherein at the outlet of each column a detector for the analysis of the components at the outlet is located.
 7. The chromatographic method according to claim 1, wherein the first cycle is preceded by a startup step, in which the columns are interconnected and wherein a larger amount of feed solution is loaded into the upstream column in comparison with an interconnected step of a cycle of the method.
 8. The chromatographic method according to claim 1, wherein the desired product is one or a group of chemical reaction products, chemical separation products, biochemical reaction products, biological products.
 9. A method for setting up a chromatographic process according to claim 1, wherein the batch step duration of the batch step is set to be the accumulated time required for recovery and regeneration of the respective column, and the batch step feed flow rate applied to the respective column in the batch step is set such that at the end of the batch step duration at the outlet of the column there is essentially no desired product elution, taking into account that in the preceding step this column has already been preloaded, and wherein the interconnected step feed flow rate is set to a desired value and the interconnected step duration is set such that at the end of the interconnected step duration at the outlet of the upstream column the desired product concentration is in desired range of the concentration at its inlet, with the proviso that there is essentially no desired product elution at the outlet of the downstream column, and wherein for the determination of the batch step feed flow rate and the interconnected step duration at least one breakthrough curve of a single column using the interconnected step feed flow rate is recorded and used, if needed at least one breakthrough curve of interconnected columns using the interconnected step feed flow rate is recorded and used, and wherein at least one breakthrough curve of a single column using a lower batch step feed flow rate than the interconnected step feed flow rate is recorded and used.
 10. The method for setting up a chromatographic process according to claim 9, wherein the following steps are carried out: (a) Calculation of the duration of the disconnected state by summing up of the durations of recovery and regeneration steps carried out with the column which is not fed with feed; (b) Determination of the elution volume breakthrough value EV₂ of two interconnected columns using the desired maximum feed flow rate Q_(feed,IC) of the interconnected state by measuring a dynamic breakthrough curve of two sequential columns DBC₂ and determining of the elution volume EV corresponding to a desired breakthrough value; (c) Determination of the elution volume breakthrough value of a single column using the feed flow rate Q_(feed,IC), wherein a full breakthrough curve DBC_(1H) is recorded, and wherein optionally the elution volume EV_(1H,1) corresponding to a desired low breakthrough value of the single column is determined; (d) Determination, from the breakthrough curve determined in step (c), of the X % breakthrough elution volume EV_(1H,X) corresponding to a high load of the single column using the desired maximum flow rate of the single column load operation; (e) Determining EV_(Y), the smaller one of the two elution volume values EV₂ of step (b) and EV_(1H,X) of step (d); (f) Determination of the preload value PL, calculated by integrating the difference of the calibrated breakthrough curve DBC_(1H) of the single column, as determined in step (c), and the calibrated breakthrough curve DBC₂ of the interconnected columns, as determined in above (b), with the upper boundary of the value determined in (e) EV_(Y), divided by the column volume of the single column according to equation 1: $\begin{matrix} {{PL} = {{\frac{1}{V_{col}} \cdot {\int_{{EV}_{{1\; H},1}}^{{EV}_{Y}}{{DBC}_{1\; H}({EV})}}} - {{{DBC}_{2}({EV})}\ {{EV}}}}} & \left( {{equation}\mspace{14mu} 1} \right) \end{matrix}$ wherein also zero can be used as lower starting point for the integration; (g) Determination of a second breakthrough value EV_(1L,1) of a single column using a lower flow rate Q_(feed,BT1L) by choosing a reduced feed flow rate, preferably a feed rate 50-90% of the feed flow rate used in step (c), and recording a full breakthrough curve DBC_(1L) and determination of the elution volume corresponding to a desired low breakthrough value; (h) Determination of the interconnected state switch time by subtracting the second breakthrough value multiplied with a safety factor Z %·EV_(1L,1) from the value determined in (e) EV_(Y) and dividing the complete term by the feed flow rate Q_(feed,IC) in the interconnected state, according to equation 2: t _(IC)=(EV_(Y) −Z %·EV_(1L,1))/Q _(feed,IC)  (equation 2); (i) Determination of the target load value TL, from the two breakthrough curves DBC₁ and DBC_(1L) of the single columns by subtracting the dead volume from the elution volume corresponding to the desired breakthrough, multiplied with a safety factor Z, and multiplying the difference with the feed concentration value, dividing said product by the column volume and subtracting PL from this value, according to equation 3: TL=((Z %·EV_(1L,1) −V _(dead))·c _(feed))/V _(col)·PL  (equation 3); (j) Determination of the feed flow rate of the disconnected state Q_(feed,B), according to equation 4 by multiplying the target load value TL with the column volume, dividing by the feed concentration, and dividing by the duration of the disconnected state t_(B) according to equation 4 Q _(feed,B)=(TL·V _(col))/(c _(feed) ·t _(B))  (equation 4).
 11. The method for setting up a chromatographic process according to claim 9, wherein (k) for the determination of the startup time t_(startup) the difference of the second breakthrough value multiplied with a safety margin Z %·EV_(1L,1) and the dead volume of a single column V_(dead) by the feed flow rate Q_(feed,IC) and adding this value to the duration of the interconnected step t_(IC)), according to equation 5 t _(startup) =t _(IC)+(Z %·EV_(1L,1) −V _(dead))/Q _(feed,IC)=(EV_(Y) −V _(dead))/Q _(feed,IC)  (equation 5) and/or wherein (l) for the determination of the duration of the interconnected state washing step t_(wash,IC) that follows the interconnected phase loading step at least the dead volume V_(dead) is divided by the flow rate Q_(wash,IC) according to equation 6: t _(wash,IC) =k·V _(dead) /Q _(wash,IC)  (equation 6) , with k as rational number≧1.
 12. A method for control and/or monitoring and/or optimization of a chromatographic process as defined in claim 1, in which the process comprises 2 columns which are operated, alternatingly, in interconnected and disconnected state, and in which the columns switch positions after such a sequence of interconnected and disconnected state, and wherein downstream of each column a detector is located capable of detecting the desired product and/or impurities when passing the detector, wherein the method comprises the following elements: (a) Measuring the disconnected areas A_(peaki) corresponding to the effluents of all columns in a product elution position in a disconnected state in at least one cycle or parts of one cycle using the detectors at the columns outlets; and/or Measuring the interconnected areas A_(ICiU) corresponding to the effluents of all columns in an interconnected state upstream and/or downstream positions in at least one cycle or parts of one cycle using the detectors at the columns outlets; and (c) Comparing the disconnected areas A_(peaki) among the two detectors; and/or (d) Comparing the interconnected areas A_(ICiU) among the two detectors; and (e) Comparing the values of the disconnected areas A_(peaki) comparison in step (c) and/or the interconnected areas A_(ICiU) comparison in step (d) among two detectors in order to quantify the degree of the signal magnitude difference and using this difference for control and/or monitoring and/or optimization of at least one process parameter.
 13. The method for control and/or monitoring and/or optimization of a chromatographic process according to claim 12, wherein the method comprises the following elements: (a) Measuring the disconnected areas A_(peaki) corresponding to the effluents of all columns in a product elution position in a disconnected state in at least one cycle or parts of one cycle using the detectors at the columns outlets; and (c) Comparing the disconnected areas A_(peaki) among the at least two detectors; and (e) Comparing the values of the disconnected areas A_(peaki) comparison in step (c) in order to quantify the degree of the signal magnitude difference and using this difference for control and/or monitoring and/or optimization of at least one process parameter.
 14. The method for control and/or monitoring and/or optimization of a chromatographic process according to claim 12, wherein the method comprises the following elements: (b) Measuring the interconnected areas A_(ICiU) corresponding to the effluents of all columns in an interconnected state upstream and/or downstream positions in at least one cycle or parts of one cycle using the detectors at the columns outlets; and (d) Comparing the interconnected areas A_(ICiU) among the at least two detectors; and (e) Comparing the values of the interconnected areas A_(ICiU) comparison in step (d) among at least two detectors in order to quantify the degree of the signal magnitude difference and using this difference for control and/or monitoring and/or optimization of at least one process parameter.
 15. A computer program product, on a storage medium, adapted to automatically design and/or control and/or monitor and/or optimize a process according to claim 1 based on a numerical evaluation, a differential and/or ratio-based numerical evaluation, of signals from the detectors or from offline analyses, based on a numerical evaluation, a differential and/or ratio-based numerical evaluation, of signals from the detectors or from offline analyses of at least two cycles.
 16. The chromatographic method according to claim 1, wherein in the interconnected step the second flow rate is adapted to be at least 10% larger than the first flow rate.
 17. The chromatographic method according to claim 1, wherein in the interconnected step the second flow rate is adapted to be at least 25% larger than the first flow rate.
 18. The chromatographic method according to claim 1, wherein in the interconnected step the second flow rate is adapted to be 1.5-4.0 times larger than the first flow rate.
 19. The chromatographic method according to claim 1, wherein in the interconnected step the second flow rate and/or the interconnected timespan are adapted such that at the end of interconnected timespan the feed concentration at the outlet of the upstream column is in the range of 30-90% of the feed concentration at the inlet of the upstream column, with the proviso that the values of the second flow rate and/or the interconnected timespan are adapted such that at the end of the interconnected timespan at the outlet of the downstream column the feed concentration is below a breakthrough value of 1-2.5%, and that the elution volume corresponding to this breakthrough value is multiplied with a safety factor in the range of 60-90%.
 20. The chromatographic method according to claim 1, wherein the batch timespan is chosen to be the accumulated time required for the recovery and regeneration of the respective column, wherein this accumulated time is given by the accumulated time required for the steps of: (i) washing with solvent and/or buffer under conditions that the product is not released from the stationary phase; (ii) elution with solvent and/or buffer under conditions that the product is released from the stationary phase; (iii) cleaning in place using a solvent and/or buffer to release everything from the stationary phase; (iv) equilibration, by using the solvent and/or buffer under conditions similar or the same as in the subsequent process steps.
 21. The chromatographic method according to claim 1, wherein the columns are affinity chromatography material loaded columns, wherein further the chromatographic stationary phase is in the form of particles in the form of beads and/or membranes and/or monoliths.
 22. The chromatographic method according to claim 1, wherein at the outlet of each column a detector for the analysis of the components at the outlet is located, both detectors being of the same type, wherein this is a detector selected from one or a combination of the following detectors: UV detector, visible light detector, IR detector, fluorescence detector, light scattering detector, refractive index detector, pH detector, conductivity detector, at-line HPLC analysis, or mass spectrometry detector.
 23. The chromatographic method according to claim 1, wherein the last cycle is followed by a shutdown step, in which the two columns are disconnected, and both columns are subjected to product recovery and column regeneration.
 24. The chromatographic method according to claim 1, wherein the desired product is one or a group of chemical reaction products, chemical separation products, biochemical reaction products, biological products, wherein the reaction products are natural products, metals, antibodies, antibody fragments, fusion proteins, recombinant glycoproteins, and/or plasma proteins, or derivatives and/or combinations and/or mixtures thereof.
 24. A method for setting up a chromatographic process according to claim 1, wherein the batch step duration of the batch step is set to be the accumulated time required for recovery and regeneration of the respective column, and the batch step feed flow rate applied to the respective column in the batch step is set such that at the end of the batch step duration at the outlet of the column there is essentially no desired product elution, taking into account that in the preceding step this column has already been preloaded, and wherein the interconnected step feed flow rate is set to a desired value and the interconnected step duration is set such that at the end of the interconnected step duration at the outlet of the upstream column the desired product concentration is in desired range of 30-90%, of the concentration at its inlet, with the proviso that there is essentially no desired product elution at the outlet of the downstream column, and wherein for the determination of the batch step feed flow rate and the interconnected step duration at least one breakthrough curve of a single column using the interconnected step feed flow rate is recorded and used, if needed at least one breakthrough curve of interconnected columns using the interconnected step feed flow rate is recorded and used, and wherein at least one breakthrough curve of a single column using a lower batch step feed flow rate than the interconnected step feed flow rate is recorded and used, wherein the lower batch step feed flow rate for the determination of this breakthrough curve is chosen to be 50-90% of the interconnected step feed flow rate.
 25. The method for setting up a chromatographic process according to claim 9, wherein the following steps are carried out, in the given order: (a) Calculation of the duration of the disconnected state t_(B) by summing up of the durations of recovery and regeneration steps carried out with the column which is not fed with feed; (b) Determination of the elution volume breakthrough value EV₂ of two interconnected columns using the desired maximum feed flow rate Q_(feed,IC) of the interconnected state by measuring a dynamic breakthrough curve of two sequential columns DBC₂ and determining of the elution volume EV corresponding to a desired breakthrough value, namely 1% breakthrough up to 5% breakthrough, with respect to the feed concentration, optionally multiplied with a safety factor, in the range between 0.5 and 1.0; (c) Determination of the elution volume breakthrough value of a single column using the feed flow rate Q_(feed,IC), wherein a full breakthrough curve DBC_(1H) is recorded, and wherein optionally the elution volume EV_(1H,1) corresponding to a desired low breakthrough value of the single column, namely 1% breakthrough with respect to the feed concentration is determined; (d) Determination, from the breakthrough curve determined in step (c), of the X % breakthrough elution volume EV_(1H,X) corresponding to a high load of the single column using the desired maximum flow rate of the single column load operation, wherein X is a number between 50 and 90%; (e) Determining EV_(Y), the smaller one of the two elution volume values EV₂ of step (b) and EV_(1H,X) of step (d); (f) Determination of the preload value PL, calculated by integrating the difference of the calibrated breakthrough curve DBC_(1H) of the single column, as determined in step (c), and the calibrated breakthrough curve DBC₂ of the interconnected columns, as determined in above (b), with the upper boundary of the value determined in (e) EV_(Y), divided by the column volume of the single column according to equation 1: $\begin{matrix} {{PL} = {{\frac{1}{V_{col}} \cdot {\int_{{EV}_{{1\; H},1}}^{{EV}_{Y}}{{DBC}_{1\; H}({EV})}}} - {{{DBC}_{2}({EV})}\ {{EV}}}}} & \left( {{equation}\mspace{14mu} 1} \right) \end{matrix}$ wherein also zero can be used as lower starting point for the integration; (g) Determination of a second breakthrough value EV_(1L,1) of a single column using a lower flow rate Q_(feed,BT1L) by choosing a reduced feed flow rate, namely a feed rate 50-90% of the feed flow rate used in step (c), and recording a full breakthrough curve DBC_(1L) and determination of the elution volume corresponding to a desired low breakthrough value, namely 1% breakthrough with respect to the feed concentration, choosing the same value as in the determination of the single column breakthrough value at the higher flow rate; (h) Determination of the interconnected state switch t_(IC) time by subtracting the second breakthrough value multiplied with a safety factor Z %·EV_(1L,1) from the value determined in (e) EV_(Y) and dividing the complete term by the feed flow rate Q_(feed,IC) in the interconnected state, according to equation 2: t _(IC)=(EV_(Y) −Z %·EV_(1L,1))/Q _(feed,IC)  (equation 2); (i) Determination of the target load value TL, from the two breakthrough curves DBC₁ and DBC_(1L) of the single columns by subtracting the dead volume from the elution volume corresponding to the desired breakthrough, namely of a breakthrough of 1%, multiplied with a safety factor Z, and multiplying the difference with the feed concentration value, dividing said product by the column volume and subtracting PL from this value, according to equation 3: TL=((Z %·EV_(1L,1) −V _(dead))·c _(feed))/V _(col)−PL  (equation 3); (j) Determination of the feed flow rate of the disconnected state Q_(feed,B), according to equation 4 by multiplying the target load value TL with the column volume, dividing by the feed concentration, and dividing by the duration of the disconnected state t_(B) according to equation 4 Q _(feed,B) =TL·V _(col))/(c _(feed) ·t _(B))  (equation 4).
 26. The method for setting up a chromatographic process according to claim 9, wherein (k) for the determination of the startup time t_(startup) difference of the second breakthrough value multiplied with a safety margin Z %·EV_(1L,1) and the dead volume of a single column V_(dead) by the feed flow rate and adding this value to the duration of the interconnected step, according to equation 5 t _(startup) =t _(IC)+(Z %·EV_(1L,1) −V _(dead))/Q _(feed,IC)=(EV_(Y) −V _(dead))Q _(feed,IC)  (equation 5) and/or wherein (l) for the determination of the duration of the interconnected state washing step that follows the interconnected phase loading step at least the dead volume V_(dead) is divided by the flow rate (Q_(wash,IC)) according to equation 6: t _(wash,IC) =k·V _(dead) /Q _(wash,IC)  (equation 6) , with k as rational number≧1.
 27. A method for control and/or monitoring and/or optimization of a chromatographic process as defined in claim 1, in which the process comprises 2 columns which are operated, alternatingly, in interconnected and disconnected state, and in which the columns switch positions after such a sequence of interconnected and disconnected state, and wherein downstream of each column a detector is located capable of detecting the desired product and/or impurities when passing the detector, wherein the method comprises the following elements: (a) Measuring the disconnected areas A_(peaki) corresponding to the effluents of all columns in a product elution position in a disconnected state in at least one cycle or parts of one cycle using the detectors at the columns outlets; and/or Measuring the interconnected areas A_(ICiU) corresponding to the effluents of all columns in an interconnected state upstream and/or downstream positions in at least one cycle or parts of one cycle using the detectors at the columns outlets; and (c) Comparing the disconnected areas A_(peaki) among the two detectors, by calculating the ratios between disconnected areas A_(peaki) of the detectors; and/or (d) Comparing the interconnected areas A_(ICiU) among the two detectors, by calculating the ratios between interconnected areas A_(ICiU) of the detectors; and (e) Comparing the values of the disconnected areas A_(peaki) comparison in step (c) and/or the interconnected areas A_(ICiU) comparison in step (d) among two detectors in order to quantify the degree of the signal magnitude difference and using this difference for control and/or monitoring and/or optimization of at least one process parameter.
 28. The method for control and/or monitoring and/or optimization of a chromatographic process according to claim 12, wherein the method comprises the following elements: (a) Measuring the disconnected areas A_(peaki) corresponding to the effluents of all columns in a product elution position in a disconnected state in at least one cycle or parts of one cycle using the detectors at the columns outlets; and (c) Comparing the disconnected areas A_(peaki) among the two detectors, by calculating the ratios between disconnected areas A_(peaki) of the detectors; and (e) Comparing the values of the disconnected areas A_(peaki) comparison in step (c) in order to quantify the degree of the signal magnitude difference and using this difference for control and/or monitoring and/or optimization of at least one process parameter.
 29. The method for control and/or monitoring and/or optimization of a chromatographic process according to claim 12, wherein the method comprises the following elements: Measuring the interconnected areas A_(ICiU) corresponding to the effluents of all columns in an interconnected state upstream and/or downstream positions in at least one cycle or parts of one cycle using the detectors at the columns outlets; and (d) Comparing the interconnected areas A_(ICiU) among the two detectors, by calculating the ratios between interconnected areas A_(ICiU) of the detectors; and (e) Comparing the values of the interconnected areas A_(ICiU) comparison in step (d) among two detectors in order to quantify the degree of the signal magnitude difference and using this difference for control and/or monitoring and/or optimization of at least one process parameter. 